β-Adrenergic signaling accelerates and synchronizes cardiac ryanodine receptor response to a single L-type Ca 2+ channel
Open Access
- 20 October 2009
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 106 (42), 18028-18033
- https://doi.org/10.1073/pnas.0906560106
Abstract
As the most prototypical G protein-coupled receptor, β-adrenergic receptor (βAR) regulates the pace and strength of heart beating by enhancing and synchronizing L-type channel (LCC) Ca2+ influx, which in turn elicits greater sarcoplasmic reticulum (SR) Ca2+ release flux via ryanodine receptors (RyRs). However, whether and how βAR-protein kinase A (PKA) signaling directly modulates RyR function remains elusive and highly controversial. By using unique single-channel Ca2+ imaging technology, we measured the response of a single RyR Ca2+ release unit, in the form of a Ca2+ spark, to its native trigger, the Ca2+ sparklet from a single LCC. We found that acute application of the selective βAR agonist isoproterenol (1 μM, ≤20 min) increased triggered spark amplitude in an LCC unitary current-independent manner. The increased ratio of Ca2+ release flux underlying a Ca2+ spark to SR Ca2+ content indicated that βAR stimulation helps to recruit additional RyRs in synchrony. Quantification of sparklet-spark kinetics showed that βAR stimulation synchronized the stochastic latency and increased the fidelity (i.e., chance of hit) of LCC-RyR intermolecular signaling. The RyR modulation was independent of the increased SR Ca2+ content. The PKA antagonists Rp-8-CPT-cAMP (100 μM) and H89 (10 μM) both eliminated these effects, indicating that βAR acutely modulates RyR activation via the PKA pathway. These results demonstrate unequivocally that RyR activation by a single LCC is accelerated and synchronized during βAR stimulation. This molecular mechanism of sympathetic regulation will permit more fundamental studies of altered βAR effects in cardiovascular diseases.Keywords
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