Tumour microvesicles contain retrotransposon elements and amplified oncogene sequences
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Open Access
- 1 February 2011
- journal article
- research article
- Published by Springer Science and Business Media LLC in Nature Communications
- Vol. 2 (1), 1-9
- https://doi.org/10.1038/ncomms1180
Abstract
Tumour cells release an abundance of microvesicles containing a selected set of proteins and RNAs. Here, we show that tumour microvesicles also carry DNA, which reflects the genetic status of the tumour, including amplification of the oncogene c-Myc. We also find amplified c-Myc in serum microvesicles from tumour-bearing mice. Further, we find remarkably high levels of retrotransposon RNA transcripts, especially for some human endogenous retroviruses, such as LINE-1 and Alu retrotransposon elements, in tumour microvesicles and these transposable elements could be transferred to normal cells. These findings expand the nucleic acid content of tumour microvesicles to include: elevated levels of specific coding and non-coding RNA and DNA, mutated and amplified oncogene sequences and transposable elements. Thus, tumour microvesicles contain a repertoire of genetic information available for horizontal gene transfer and potential use as blood biomarkers for cancer.Keywords
This publication has 59 references indexed in Scilit:
- MicroRNA involvement in glioblastoma pathogenesisBiochemical and Biophysical Research Communications, 2009
- Proteomic and immunologic analyses of brain tumor exosomesThe FASEB Journal, 2008
- Glioblastoma microvesicles transport RNA and proteins that promote tumour growth and provide diagnostic biomarkersNature, 2008
- Comprehensive genomic characterization defines human glioblastoma genes and core pathwaysNature, 2008
- Replication licensing and cancer — a fatal entanglement?Nature Reviews Cancer, 2008
- Trex1 Prevents Cell-Intrinsic Initiation of AutoimmunityCell, 2008
- LINE-1 ORF1 protein enhances Alu SINE retrotranspositionGene, 2008
- MicroRNA signatures of tumor-derived exosomes as diagnostic biomarkers of ovarian cancerGynecologic Oncology, 2008
- Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cellsNature, 2007
- Hypermethylation of CpG island loci and hypomethylation of LINE‐1 and Alu repeats in prostate adenocarcinoma and their relationship to clinicopathological featuresThe Journal of Pathology, 2006