Simultaneous Analysis of Strychnine and Brucine and their Major Metabolites by Liquid Chromatography-Electrospray Ion Trap Mass Spectrometry
Open Access
- 14 March 2012
- journal article
- Published by Oxford University Press (OUP) in Journal of Analytical Toxicology
- Vol. 36 (3), 171-176
- https://doi.org/10.1093/jat/bks004
Abstract
A liquid chromatography–electrospray ionization–ion trap mass spectrometry (LC–ESI–ITMS) method was developed for the simultaneous analysis of strychnine, brucine and their major metabolites. Strychnine and brucine were individually incubated with rat liver S9 fraction. The incubation samples were pooled together and analyzed with LC–ESI–ITMS in positive ion and full-scan detection mode. The calibration curves of strychnine and brucine in rat liver showed good linearity in ranges of 0.020 to 8.0 µg/mL for strychnine and 0.020 to 8.5 µg/mL for brucine. The limits of detections were both 0.008 µg/mL and the recoveries were 88.3 and 83.2% for strychnine and brucine, respectively. Two metabolites were identified as strychnine N-oxide and brucine N-oxide by comparing the molecular mass, retention time, full-scan mass spectra, tandem MS and MS3 spectra with those of strychnine and brucine. The developed method provided high sensitivity and selectivity for the determination of poisonous alkaloids and their major metabolites and can be applied in the determination of samples in forensic and clinically toxicological cases.Keywords
This publication has 10 references indexed in Scilit:
- Metabolomic analysis of uremic toxins by liquid chromatography/electrospray ionization-tandem mass spectrometryJournal of Chromatography B, 2010
- Development and validation of a liquid chromatography–tandem mass spectrometry assay for the simultaneous quantitation of microcystin-RR and its metabolites in fish liverJournal of Chromatography A, 2010
- Development and validation of high-throughput liquid chromatography–tandem mass spectrometric method for simultaneous quantification of Clopidogrel and its metabolite in human plasmaJournal of Chromatography B, 2010
- Determination of strychnine and brucine in rat plasma using liquid chromatography electrospray ionization mass spectrometryJournal of Pharmaceutical and Biomedical Analysis, 2009
- Simultaneous determination of thirteen plant alkaloids in a human specimen by SPE and HPLCJournal of Separation Science, 2008
- Determination of Strychnine in Human Blood using Solid-Phase Extraction and GC-EI-MSJournal of Analytical Toxicology, 2005
- About the toxicity of some Strychnos species and their alkaloidsToxicon, 2004
- A capsule review of recent studies on the application of mass spectrometry in the analysis of Chinese medicinal herbsJournal of Mass Spectrometry, 2002
- Simultaneous quantitative cassette analysis of drugs and detection of their metabolites by high performance liquid chromatography/ion trap mass spectrometryRapid Communications in Mass Spectrometry, 2000
- Species difference in metabolism of strychnine with liver microsomes of mice, rats, guinea pigs, rabbits and dogs.Journal of Pharmacobio-Dynamics, 1990