Effect of low level diode laser irradiation of human oral mucosa fibroblasts in vitro
- 1 January 1994
- journal article
- Published by Wiley in Lasers in Surgery and Medicine
- Vol. 14 (4), 347-354
- https://doi.org/10.1002/lsm.1900140407
Abstract
The effects of low level laser (LLL) irradiation on the proliferation of human buccal fibroblasts were studied. A standardized LLL set‐up was developed (812 nm, 4.5 ± 0.5 mW/cm2). Cultures in petridishes were divided into eight groups (1 group served as control). On day 6 after seeding, routine growth medium was replaced with PBS for 1/2 hour. At the beginning of this period, LLL irradiation was performed for 0, 1, 3, 10, 32, 100, 316, or 1,000 seconds, respectively—corresponding to the radiant exposures 0, 4.5, 13.5, 45, 144, 450, 1,422, 4,500 mJ/cm2. Subsequently the cells received 3H‐dT in fresh medium for 16 hours DNA‐incorporation. Scintillations from tritium and total protein concentration per culture dish were determined. The individual 3H‐cpm/protein‐concentration ratios were calculated in % of control. Three experiments were performed (N = 151). Following LLL exposure the H‐cpm/protein ratio was increased with maximum cpm/protein ratio (132.5% ± 10.6% SEM) in the group receiving 450 mJ/cm2 (P < 0.03 nonparametric Kruskal Wallis one‐way ANOVA‐test). This study demonstrated an increased incorporation on tritiated thymidine in cultured human oral fibroblasts following LLL exposure and suggests that LLL irradiation can induce increased DNA Synthesis.Keywords
This publication has 42 references indexed in Scilit:
- Power density and exposure time of He‐Ne laser irradiation are more important than total energy dose in photo‐biomodulation of human fibroblasts in vitroLasers in Surgery and Medicine, 1992
- A possible explanation of laser-induced stimulation and damage of cell culturesJournal of Photochemistry and Photobiology B: Biology, 1991
- Effect of low level laser therapy on wound healing after palatal surgery in Beagle dogsLasers in Surgery and Medicine, 1991
- Verbessert das Licht des Soft-Lasers bzw. des kombinierten Soft- und Middlepower-Lasers die Wundheilung bei Ratten? Weitergehende Untersuchungen - Does the Light from Soft Lasers or Combined Soft and Middle-Power Lasers Improve Wound Healing in Rats? - Further StudiesBiomedizinische Technik/Biomedical Engineering, 1990
- Biostimulation der Wundheilung bei Ratten durch kombinierten Soft- und Middlepower-Laser - Biostimulation of Wound Healing in Rats with combined Soft- and Middle-Power LaserBiomedizinische Technik/Biomedical Engineering, 1990
- The effect of low-energy infra-red laser radiation on wound healing in ratsBritish Journal of Oral and Maxillofacial Surgery, 1988
- Demonstration of elevated type I and type III procollagen mRNA levels in cutaneous wounds treated with helium-neon laser: Proposed mechanism for enhanced wound healingBiochemical and Biophysical Research Communications, 1986
- Control of connective tissue metabolism by lasers: Recent developments and future prospectsJournal of the American Academy of Dermatology, 1984
- Fluorescent light-induced chromosome damage in human IMR-90 fibroblasts role of hydrogen peroxide and related free radicalsMutation research. Reviews in mutation research, 1980
- Photostimulation of ATP production, in cell-free extracts of insect integumentCellular and Molecular Life Sciences, 1980