Identification of the Candida albicans Cap1p Regulon
Open Access
- 1 June 2009
- journal article
- research article
- Published by American Society for Microbiology in Eukaryotic Cell
- Vol. 8 (6), 806-820
- https://doi.org/10.1128/ec.00002-09
Abstract
Cap1p, a transcription factor of the basic region leucine zipper family, regulates the oxidative stress response (OSR) in Candida albicans . Alteration of its C-terminal cysteine-rich domain (CRD) results in Cap1p nuclear retention and transcriptional activation. To better understand the function of Cap1p in C. albicans , we used genome-wide location profiling (chromatin immunoprecipitation-on-chip) to identify its transcriptional targets in vivo. A triple-hemagglutinin (HA 3 ) epitope was introduced at the C terminus of wild-type Cap1p (Cap1p-HA 3 ) or hyperactive Cap1p with an altered CRD (Cap1p-CSE-HA 3 ). Location profiling using whole-genome oligonucleotide tiling microarrays identified 89 targets bound by Cap1p-HA 3 or Cap1p-CSE-HA 3 (the binding ratio was at least twofold; P ≤ 0.01). Strikingly, Cap1p binding was detected not only at the promoter region of its target genes but also at their 3′ ends and within their open reading frames, suggesting that Cap1p may associate with the transcriptional or chromatin remodeling machinery to exert its activity. Overrepresented functional groups of the Cap1p targets ( P ≤ 0.02) included 11 genes involved in the OSR ( CAP1 , GLR1 , TRX1 , SOD1 , CAT1 , and others), 13 genes involved in response to drugs ( PDR16 , MDR1 , FLU1 , YCF1 , FCR1 , and others), 4 genes involved in phospholipid transport ( PDR16 , GIT1 , RTA2 , and orf19.932), and 3 genes involved in the regulation of nitrogen utilization ( GST3 , orf19.2693, and orf19.3121), suggesting that Cap1p has other cellular functions in addition to the OSR. Bioinformatic analyses of the bound sequences suggest that Cap1p recognizes the DNA motif 5′-MTKASTMA. Finally, transcriptome analyses showed that increased expression generally accompanies Cap1p binding at its targets, indicating that Cap1p functions as a transcriptional activator.This publication has 74 references indexed in Scilit:
- Mutations in the multi‐drug resistance regulator MRR1, followed by loss of heterozygosity, are the main cause of MDR1 overexpression in fluconazole‐resistant Candida albicans strainsMolecular Microbiology, 2008
- A Gain-of-Function Mutation in the Transcription Factor Upc2p Causes Upregulation of Ergosterol Biosynthesis Genes and Increased Fluconazole Resistance in a Clinical Candida albicans IsolateEukaryotic Cell, 2008
- Genomewide Location Analysis of Candida albicans Upc2p, a Regulator of Sterol Metabolism and Azole Drug ResistanceEukaryotic Cell, 2008
- Genotypic Evolution of Azole Resistance Mechanisms in Sequential Candida albicans IsolatesEukaryotic Cell, 2007
- SCOPE: a web server for practical de novo motif discoveryNucleic Acids Research, 2007
- Transcriptional Regulation of MDR1 , Encoding a Drug Efflux Determinant, in Fluconazole-Resistant Candida albicans Strains through an Mcm1p Binding SiteEukaryotic Cell, 2006
- Genome-wide analysis of estrogen receptor binding sitesNature Genetics, 2006
- Identifying transcription factor functions and targets by phenotypic activationProceedings of the National Academy of Sciences, 2006
- TFIIA Plays a Role in the Response to Oxidative StressEukaryotic Cell, 2006
- Multiple cis -Acting Sequences Mediate Upregulation of the MDR1 Efflux Pump in a Fluconazole-Resistant Clinical Candida albicans IsolateAntimicrobial Agents and Chemotherapy, 2006