Quantitative imaging of ischemic stroke through thinned skull in mice with Multi Exposure Speckle Imaging
Open Access
- 16 July 2010
- journal article
- Published by Optica Publishing Group in Biomedical Optics Express
- Vol. 1 (1), 246-259
- https://doi.org/10.1364/boe.1.000246
Abstract
Laser Speckle Contrast Imaging (LSCI) has become a widely used technique to image cerebral blood flow in vivo. However, the quantitative accuracy of blood flow changes measured through the thin skull has not been investigated thoroughly. We recently developed a new Multi Exposure Speckle Imaging (MESI) technique to image blood flow while accounting for the effect of scattering from static tissue elements. In this paper we present the first in vivo demonstration of the MESI technique. The MESI technique was used to image the blood flow changes in a mouse cortex following photothrombotic occlusion of the middle cerebral artery. The Multi Exposure Speckle Imaging technique was found to accurately estimate flow changes due to ischemia in mice brains in vivo. These estimates of these flow changes were found to be unaffected by scattering from thinned skull.Keywords
This publication has 29 references indexed in Scilit:
- Laser speckle contrast imaging in biomedical opticsJournal of Biomedical Optics, 2010
- Optical imaging of the spatiotemporal dynamics of cerebral blood flow and oxidative metabolism in the rat barrel cortexEuropean Journal of Neuroscience, 2004
- Laser speckle imaging for monitoring blood flow dynamics in the in vivo rodent dorsal skin fold modelMicrovascular Research, 2004
- Mouse Model of Microembolic Stroke and ReperfusionStroke, 2004
- Spatiotemporal Quantification of Cerebral Blood Flow during Functional Activation in Rat Somatosensory Cortex using Laser-Speckle FlowmetryJournal of Cerebral Blood Flow & Metabolism, 2004
- Laser Doppler, speckle and related techniques for blood perfusion mapping and imagingPhysiological Measurement, 2001
- Dynamic Imaging of Cerebral Blood Flow Using Laser SpeckleJournal of Cerebral Blood Flow & Metabolism, 2001
- Measurement of microcirculation in optic nerve head by laser speckle flowgraphy in normal volunteersAmerican Journal of Ophthalmology, 2000
- Measuring the steady-state value and the dynamics of the skin blood flow using the non-contact laser speckle methodMedical Engineering & Physics, 1994
- Flow visualization by means of single-exposure speckle photographyOptics Communications, 1981