Proteomic profiling of cerebrospinal fluid identifies prostaglandin D2 synthase as a putative biomarker for pediatric medulloblastoma: A pediatric brain tumor consortium study
- 7 January 2011
- journal article
- research article
- Published by Wiley in Proteomics
- Vol. 11 (5), 935-943
- https://doi.org/10.1002/pmic.201000198
Abstract
The aims of this study were to demonstrate the feasibility of centrally collecting and processing high‐quality cerebrospinal fluid (CSF) samples for proteomic studies within a multi‐center consortium and to identify putative biomarkers for medulloblastoma in CSF. We used 2‐DE to investigate the CSF proteome from 33 children with medulloblastoma and compared it against the CSF proteome from 25 age‐matched controls. Protein spots were subsequently identified by a combination of in‐gel tryptic digestion and MALDI‐TOF TOF MS analysis. On average, 160 protein spots were detected by 2‐DE and 76 protein spots corresponding to 25 unique proteins were identified using MALDI‐TOF. Levels of prostaglandin D2 synthase (PGD2S) were found to be six‐fold decreased in the tumor samples versus control samples (p78 and Asn87. Total PGD2S levels are reduced six‐fold in the CSF of children with medulloblastoma most likely representing a host response to the presence of the tumor. In addition, our results demonstrate the feasibility of performing proteomic studies on CSF samples collected from patients at multiple institutions within the consortium setting.Keywords
Funding Information
- NIH (U01 CA81457)
- PBTC
- American Lebanese Syrian Associated Charities
- The Childhood Brain Tumor Foundation
- The Becca's Run Fund
- The Schlobohm Family
- Friends of Ian
- NIH core grants (1P30HD40677, 5R24 HD050846)
This publication has 38 references indexed in Scilit:
- Sample size determination in clinical proteomic profiling experiments using mass spectrometry for class comparisonProteomics, 2008
- Increased Apolipoprotein E Gene Expression and Protein Concentration in Lung Cancer Tissue Do Not Contribute to the Clinical Assessment of Non-small Cell Lung Cancer PatientsArchives of Medical Research, 2008
- Functional Clustering of Metastasis Proteins Describes Plastic Adaptation Resources of Breast-Cancer Cells to New MicroenvironmentsJournal of Proteome Research, 2008
- Protein Biomarker Identification in the CSF of Patients With CNS LymphomaJournal of Clinical Oncology, 2008
- Biological Variation of the Platelet Proteome in the Elderly Population and Its Implication for Biomarker ResearchMolecular & Cellular Proteomics, 2008
- An improved LC–MS/MS method for the quantification of prostaglandins E2 and D2 production in biological fluidsAnalytical Biochemistry, 2008
- Proteomic Identification of Biomarkers in the Cerebrospinal Fluid (CSF) of Astrocytoma PatientsJournal of Proteome Research, 2007
- Involvement of apolipoprotein E in glioblastoma: immunohistochemistry and clinical outcomeNeuroReport, 2003
- The Human Plasma ProteomeMolecular & Cellular Proteomics, 2002
- Purification and Chemical Characterization of β‐Trace Protein from Human Cerebrospinal Fluid: Its Identification as Prostaglandin D SynthaseJournal of Neurochemistry, 1993