Application of a Bacteriophage Lysin To Disrupt Biofilms Formed by the Animal Pathogen Streptococcus suis
- 1 December 2011
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 77 (23), 8272-8279
- https://doi.org/10.1128/aem.05151-11
Abstract
Bacterial biofilms are crucial to the pathogenesis of many important infections and are difficult to eradicate. Streptococcus suis is an important pathogen of pigs, and here the biofilm-forming ability of 32 strains of this species was determined. Significant biofilms were completely formed by 10 of the strains after 60 h of incubation, with exopolysaccharide production in the biofilm significantly higher than that in the corresponding planktonic cultures. S. suis strain SS2-4 formed a dense biofilm, as revealed by scanning electron microscopy, and in this state exhibited increased resistance to a number of antibiotics (ampicillin, amoxicillin, ciprofloxacin, kanamycin, and rifampin) compared to that of planktonic cultures. A bacteriophage lysin, designated LySMP, was used to attack biofilms alone and in combination with antibiotics and bacteriophage. The results demonstrated that the biofilms formed by S. suis, especially strains SS2-4 and SS2-H, could be dispersed by LySMP and with >80% removal compared to a biofilm reduction by treatment with either antibiotics or bacteriophage alone of less than 20%; in addition to disruption of the biofilm structure, the S. suis cells themselves were inactivated by LySMP. The efficacy of LySMP was not dose dependent, and in combination with antibiotics, it acted synergistically to maximize dispersal of the S. suis biofilm and inactivate the released cells. These data suggest that bacteriophage lysin could form part of an effective strategy to treat S. suis infections and represents a new class of antibiofilm agents.Keywords
This publication has 41 references indexed in Scilit:
- A colorimetric microtiter plate method for assessment of phage effect on Pseudomonas aeruginosa biofilmJournal of Microbiological Methods, 2008
- Fluorescence Correlation Spectroscopy To Study Diffusion and Reaction of Bacteriophages inside BiofilmsApplied and Environmental Microbiology, 2008
- Bacteriophages: an appraisal of their role in the treatment of bacterial infectionsInternational Journal of Antimicrobial Agents, 2007
- Dispersing biofilms with engineered enzymatic bacteriophageProceedings of the National Academy of Sciences of the United States of America, 2007
- Lytic Activity of Recombinant Bacteriophage φ11 and φ12 Endolysins on Whole Cells and Biofilms of Staphylococcus aureusApplied and Environmental Microbiology, 2007
- Using Bacteriophages To Reduce Formation of Catheter-Associated Biofilms byStaphylococcus epidermidisAntimicrobial Agents and Chemotherapy, 2006
- Bacteriophage T4 multiplication in a glucose-limited Escherichia coli biofilmCanadian Journal of Microbiology, 2001
- Bacteriophage T4 multiplication in a glucose-limited Escherichia coli biofilmCanadian Journal of Microbiology, 2001
- Bacteriophage and associated polysaccharide depolymerases – novel tools for study of bacterial biofilmsJournal of Applied Microbiology, 1998
- Purification and characterization of adhesive exopolysaccharides from Pseudomonas putida and Pseudomonas fluorescensCanadian Journal of Microbiology, 1987