Tropomyosin isoforms and reagents
- 1 July 2011
- journal article
- Published by Informa UK Limited in BioArchitecture
- Vol. 1 (4), 135-164
- https://doi.org/10.4161/bioa.1.4.17897
Abstract
Tropomyosins are rod-like dimers which form head-to-tail polymers along the length of actin filaments and regulate the access of actin binding proteins to the filaments.1 The diversity of tropomyosin isoforms, over 40 in mammals, and their role in an increasing number of biological processes presents a challenge both to experienced researchers and those new to this field. The increased appreciation that the role of these isoforms expands beyond that of simply stabilizing actin filaments has lead to a surge of reagents and techniques to study their function and mechanisms of action. This report is designed to provide a basic guide to the genes and proteins and the availability of reagents which allow effective study of this family of proteins. We highlight the value of combining multiple techniques to better evaluate the function of different tm isoforms and discuss the limitations of selected reagents. Brief background material is included to demystify some of the unfortunate complexity regarding this multi-gene family of proteins including the unconventional nomenclature of the isoforms and the evolutionary relationships of isoforms between species. Additionally, we present step-by-step detailed experimental protocols used in our laboratory to assist new comers to the field and experts alike.Keywords
This publication has 117 references indexed in Scilit:
- Evolutionarily conserved surface residues constitute actin binding sites of tropomyosinProceedings of the National Academy of Sciences of the United States of America, 2011
- Tropomyosin Position on F-Actin Revealed by EM Reconstruction and Computational ChemistryBiophysical Journal, 2011
- Three's company: the fission yeast actin cytoskeletonTrends in Cell Biology, 2010
- Investigations into tropomyosin function using mouse modelsJournal of Molecular and Cellular Cardiology, 2010
- Identification of a Unique “Stability Control Region” that Controls Protein Stability of Tropomyosin: A Two-stranded α-Helical Coiled-coilJournal of Molecular Biology, 2009
- Cytoskeletal Tropomyosin Tm5NM1 Is Required for Normal Excitation–Contraction Coupling in Skeletal MuscleMolecular Biology of the Cell, 2009
- High molecular weight tropomyosins regulate osteoclast cytoskeletal morphologyBone, 2008
- Tropomyosin 4 regulates adhesion structures and resorptive capacity in osteoclastsExperimental Cell Research, 2008
- Class-Specific Interaction of Profilin and ADF Isovariants with Actin in the Regulation of Plant DevelopmentPlant Cell, 2007
- Functional Homodimers and Heterodimers of Recombinant Smooth Muscle TropomyosinBiochemistry, 2006