TBCD Links Centriologenesis, Spindle Microtubule Dynamics, and Midbody Abscission in Human Cells
Open Access
- 22 January 2010
- journal article
- research article
- Published by Public Library of Science (PLoS) in PLOS ONE
- Vol. 5 (1), e8846
- https://doi.org/10.1371/journal.pone.0008846
Abstract
Microtubule-organizing centers recruit α- and β-tubulin polypeptides for microtubule nucleation. Tubulin synthesis is complex, requiring five specific cofactors, designated tubulin cofactors (TBCs) A–E, which contribute to various aspects of microtubule dynamics in vivo. Here, we show that tubulin cofactor D (TBCD) is concentrated at the centrosome and midbody, where it participates in centriologenesis, spindle organization, and cell abscission. TBCD exhibits a cell-cycle-specific pattern, localizing on the daughter centriole at G1 and on procentrioles by S, and disappearing from older centrioles at telophase as the protein is recruited to the midbody. Our data show that TBCD overexpression results in microtubule release from the centrosome and G1 arrest, whereas its depletion produces mitotic aberrations and incomplete microtubule retraction at the midbody during cytokinesis. TBCD is recruited to the centriole replication site at the onset of the centrosome duplication cycle. A role in centriologenesis is further supported in differentiating ciliated cells, where TBCD is organized into “centriolar rosettes”. These data suggest that TBCD participates in both canonical and de novo centriolar assembly pathways.Keywords
This publication has 46 references indexed in Scilit:
- CPAP is a cell-cycle regulated protein that controls centriole lengthNature, 2009
- Functional characterization of the microtubule-binding and -destabilizing domains of CPAP and d-SAS-4Experimental Cell Research, 2008
- Plk4-Induced Centriole Biogenesis in Human CellsDevelopmental Cell, 2007
- Molecular characterization of centriole assembly in ciliated epithelial cellsThe Journal of cell biology, 2007
- Role of cofactors B (TBCB) and E (TBCE) in tubulin heterodimer dissociationExperimental Cell Research, 2007
- Possible regulation of microtubules through destabilization of tubulinTrends in Cell Biology, 2005
- Full-genome RNAi profiling of early embryogenesis in Caenorhabditis elegansNature, 2005
- Proteomic characterization of the human centrosome by protein correlation profilingNature, 2003
- Protection from Free β-Tubulin by the β-Tubulin Binding Protein Rbl2pMolecular and Cellular Biology, 2002
- Review: Postchaperonin Tubulin Folding Cofactors and Their Role in Microtubule DynamicsJournal of Structural Biology, 2001