Human large granular lymphocytes induce immunoglobulin synthesis after bone marrow transplantation
- 1 January 1987
- journal article
- research article
- Published by Wiley in European Journal of Immunology
- Vol. 17 (1), 43-47
- https://doi.org/10.1002/eji.1830170108
Abstract
Following T cell‐depleted bone marrow transplantation, helper T cell numbers remain depressed for some months. Nonetheless, functional B cells can be adoptively transferred to the recipients of such grafts, where they continue to secrete antibody. We now show that immunoglobulin production by these transferred B cells is induced by activated large granular lymphocytes (LGL) which circulate in the recipients in substantial numbers during the immediate post‐transplant period. The LGL are CD3 negative and therefore provide help in an antigen‐unlinked manner. Helper effects for autologous (donor) B cells are augmented by the addition of anti‐LFA‐2 (anti‐CD2) which appears to act by blocking recruitment of LGL inhibitory to developing B cells. In contrast antibody to the β chain of LFA‐1, which effectively reduces natural killer activity of LGL, does not influence their helper function. The peripheral blood LGL fraction thus contains both helper and cytotoxic activity, which can be distinguished by appropriate monoclonal antibodies.Keywords
This publication has 34 references indexed in Scilit:
- THE CONTRIBUTION OF LARGE GRANULAR LYMPHOCYTES TO B CELL ACTIVATION AND DIFFERENTIATION AFTER T-CELL-DEPLETED ALLOGENEIC BONE MARROW TRANSPLANTATIONTransplantation, 1986
- Natural killer cell activity following T-cell depleted allogeneic bone marrow transplantationBritish Journal of Haematology, 1986
- TRANSFER OF A FUNCTIONING HUMORAL IMMUNE SYSTEM IN TRANSPLANTATION OF T-LYMPHOCYTE-DEPLETED BONE MARROWThe Lancet, 1986
- Activation of cytolytic T lymphocyte and natural killer cell function through the T11 sheep erythrocyte binding proteinNature, 1985
- Phytohaemagglutinin activation of T cells through the sheep red blood cell receptorNature, 1985
- Effects of monoclonal antibodies to the a and β chains of the human lymphocyte function‐associated (H‐LFA‐1) antigen on T lymphocyte functionsEuropean Journal of Immunology, 1985
- Human clones with natural killer function can activate B cells and secrete B cell differentiation factorsEuropean Journal of Immunology, 1984
- ELIMINATION OF T-LYMPHOCYTES FROM HUMAN BONE MARROW WITH MONOCLONAL T-ANTIBODIES AND CYTOLYTIC COMPLEMENTBritish Journal of Haematology, 1982
- Antigen specificity and frequency of autologous and allogeneic helper T cells in the in vitro production of antibody against influenza virus by human blood lymphocytesEuropean Journal of Immunology, 1981
- Complete replacement of serum by albumin, transferrin, and soybean lipid in cultures of lipopolysaccharide-reactive B lymphocytes.The Journal of Experimental Medicine, 1978