High Probability Opening of NMDA Receptor Channels by L-Glutamate
- 24 January 1992
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 255 (5043), 470-472
- https://doi.org/10.1126/science.1346477
Abstract
Synaptic plasticity can be triggered by calcium flux into neurons through synaptically activated N-methyl-D-aspartate (NMDA) receptor channels. The amplitude and time course of the resulting intracellular calcium transient depend on the number of open NMDA receptor channels and the kinetics of their activation. Short applications of L-glutamate to outside-out patches from hippocampal neurons in the presence and absence of MK-801 revealed that about 30 percent of L-glutamate-bound channels are open at the peak of the current. This high probability of opening suggests that very few channels are required to guarantee a large, localized postsynaptic calcium transient.Keywords
This publication has 23 references indexed in Scilit:
- Activation kinetics reveal the number of glutamate and glycine binding sites on the N-methyl-d-aspartate receptorNeuron, 1991
- Quantal analysis of excitatory synaptic action and depression in hippocampal slicesNature, 1991
- Mechanisms generating the time course of dual component excitatory synaptic currents recorded in hippocampal slicesNeuron, 1990
- Channel kinetics determine the time course of NMDA receptor-mediated synaptic currentsNature, 1990
- Glycine-insensitive desensitization of NMDA responses in cultured mouse embryonic neuronsNeuron, 1990
- NMDA and non-NMDA receptors are co-localized at individual excitatory synapses in cultured rat hippocampusNature, 1989
- Postsynaptic Calcium Is Sufficient for Potentiation of Hippocampal Synaptic TransmissionScience, 1988
- Glutamate activates multiple single channel conductances in hippocampal neuronsNature, 1987
- NMDA-receptor activation increases cytoplasmic calcium concentration in cultured spinal cord neuronesNature, 1986
- Intracellular injections of EGTA block induction of hippocampal long-term potentiationNature, 1983