The occurrence of soluble and membrane-bound non-specific lipid transfer protein (sterol carrier protein 2) in rat tissues
- 1 October 1990
- journal article
- Published by Elsevier BV in Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism
- Vol. 1046 (3), 315-321
- https://doi.org/10.1016/0005-2760(90)90247-u
Abstract
The occurrence and subcellular distribution of the non-specific lipid transfer protein (nsL-TP; sterol carrier protein 2) in rat tissues was investigated by the immunoblotting technique using the affinity purified antibody against rat liver nsL-TP. Highest levels of the protein were found in the homogenates of liver, lung and adrenals, whereas it could hardly be detected in brain. In other tissues (i.e., testis, kidney, heart and intestine) the protein was present at intermediate concentrations. Analysis of subcellular fractions obtained by differential centrifugation demonstrated that in all tissues except for the liver, nsL-TP was predominantly present in the participate fractions. In the particulate fractions of all tissues, an immunoreactive 58 kDa-protein was detected. Density centrifugation of a nuclear-free homogenate from liver and testis indicated that the 58 kDa-protein did, and nsL-TP did not, cofractionate with catalase. This suggests that in these tissues the bulk of nsL-TP is extraperoxisomal. Membrane-bound nsL-TP in testis was sensitive to trypsin treatment, suggesting that it is exposed to the cytosol. Release of nsL-TP by washing the membranes with 0.1 M Na2CO3 (pH 11.5), indicated that nsL-TP is a periferal protein. It was shown by chromatofocussing that nsL-TP extracted from membrane fractions was more basic than nsL-TP present in the cytosol fraction from rat liver (isoelectric point of 8.7).Keywords
This publication has 25 references indexed in Scilit:
- Purification and characterisation of a non-specific lipid transfer protein from goat liverBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1988
- Purification of nonspecific lipid transfer protein (sterol carrier protein 2) from human liver and its deficiency in livers from patients with cerebro-hepato-renal (Zellweger) syndromeBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1987
- The role of sterol carrier protein2 and other hepatic lipid-binding proteins in bile-acid biosynthesisBiochemical Journal, 1986
- The non-specific lipid transfer protein (sterol carrier protein 2) from rat and bovine liverChemistry and Physics of Lipids, 1985
- Regulation of bile-acid synthesis. Role of sterol carrier protein2 in the biosynthesis of 7α-hydroxycholesterolBiochemical Journal, 1985
- Cytosolic modulators of activities of microsomal enzymes of cholesterol biosynthesis purification and characterization of a non-specific lipid-transfer proteinBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1983
- Increased cholesterol esterification in rat liver microsomes by purified non-specific phospholipid transfer proteinBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1982
- A new high-yield procedure for the purification of the non-specific phospholipid transfer protein from rat liverBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1981
- Two nonspecific phospholipid exchange proteins from beef liver. 1. Purification and characterizationBiochemistry, 1980
- Evidence for the participation of two soluble noncatalytic proteins in hepatic microsomal cholesterol synthesisBiochemical and Biophysical Research Communications, 1973