Marinobacter psychrophilus sp. nov., a psychrophilic bacterium isolated from the Arctic

Abstract

Strain 20041T was isolated from sea-ice of the Canadian Basin (7 ° 23′ 14″ N 14 ° 06′ 55″ W). Phylogenetic analysis based on 16S rRNA gene homology showed that strain 20041T was related to members of the genus Marinobacter and had highest 16S rRNA gene sequence similarity with the type strain of Marinobacter maritimus. Cells were Gram-negative, rod-shaped, psychrophilic and motile. The temperature range for growth was 0–22 °C, with optimum growth occurring at 16–18 °C and at approximately pH 6.0–9.0. Strain 20041T had ubiquinone-9 as the major respiratory quinone and iso-C15 : 0 2OH and/or C16 : 1 ω7c, C16 : 0, C18 : 1 ω9c and C12 : 0 3OH as major fatty acids. The genomic DNA G+C content was 55.4 mol%. On the basis of the phenotypic characteristics, phylogenetic analysis and DNA–DNA relatedness data, strain 20041T is considered to represent a novel species, for which the name Marinobacter psychrophilus sp. nov. is proposed. The type strain is 20041T (=CGMCC 1.6499T=JCM 14643T).