Cross-reactive CTL recognizing two HLA-A*02-restricted epitopes within the BK virus and JC virus VP1 polypeptides are frequent in immunocompetent individuals
- 1 June 2006
- journal article
- Published by Elsevier BV in Virology
- Vol. 350 (1), 128-136
- https://doi.org/10.1016/j.virol.2006.02.040
Abstract
Two HLA-A*02-restricted epitopes have been identified within the VP1 polypeptide of a human polyomavirus, BK virus, which is associated with polyomavirus-associated nephropathy in kidney transplant patients. Immunization of transgenic mice with recombinant modified vaccinia Ankara expressing BKV VP1 (rMVA-BKV VP1) elicited functional CTL populations recognizing the sequences LLMWEAVTV (amino acids residues 108–116, BKV VP1p108) and AITEVECFL (residues 44–52, BKV VP1p44) and cross-reactive to the previously described JC virus VP1 homologs. Flow-based analyses of PBMC from a panel of thirty healthy HLA-A*02 human volunteers indicated that the majority of these subjects harbored functional CTL populations recognizing the BKV epitopes and cross-reactive with the JCV homologs. CTL recognizing the JCV VP1p100 and JCV VP1p36 epitopes have previously been associated with prolonged survival in progressive multifocal leukoencephalopathy patients. These findings suggest that infection with BKV or JCV could potentially induce cross-protective T-cell immunity against diseases associated with these virusesKeywords
Funding Information
- National Cancer Institute
- Leukemia and Lymphoma Society (CA33572, R6145-05)
- U.S. Public Health Service (5PO1 CA30206, P01 CA30206, R01 CA77544, R21 CA104261)
This publication has 34 references indexed in Scilit:
- Cross-reactive influenza virus-specific CD8+ T cells contribute to lymphoproliferation in Epstein-Barr virus-associated infectious mononucleosisJCI Insight, 2005
- Polyomavirus BK-Specific Immunity after Kidney TransplantationTransplantation, 2004
- Dendritic Cells Pulsed with Polyomavirus BK Antigen Induce Ex Vivo Polyoma BK Virus–Specific Cytotoxic T-Cell Lines in Seropositive Healthy Individuals and Renal Transplant RecipientsJournal of the American Society of Nephrology, 2003
- Immunization with Th-CTL Fusion Peptide and Cytosine-Phosphate-Guanine DNA in Transgenic HLA-A2 Mice Induces Recognition of HIV-Infected T Cells and Clears Vaccinia Virus ChallengeThe Journal of Immunology, 2003
- Sensitive and viable identification of antigen-specific CD8+ T cells by a flow cytometric assay for degranulationJournal of Immunological Methods, 2003
- Memory CD8+ T cells vary in differentiation phenotype in different persistent virus infectionsNature Medicine, 2002
- The ER-Luminal Domain of the HCMV Glycoprotein US6 Inhibits Peptide Translocation by TAPImmunity, 1997
- Infection of Human Polyomaviruses JC and BK in Peripheral Blood Leukocytes from Immunocompetent IndividualsVirology, 1994
- Cell‐Mediated immune responses to BK virus in normal individualsJournal of Medical Virology, 1985
- Persistence of DNA Sequences of BK Virus and JC Virus in Normal Human Tissues and in Diseased TissuesThe Journal of Infectious Diseases, 1983