A simple colorimetric assay method for pyrophosphate in the presence of a 1000-fold excess of orthophosphate: Application of the method to the study of pyrophosphate metabolism in mitochondria
- 1 February 1989
- journal article
- Published by Elsevier BV in Analytical Biochemistry
- Vol. 176 (2), 390-394
- https://doi.org/10.1016/0003-2697(89)90329-1
Abstract
A sensitive colorimetric method for the assay of inorganic pyrophosphate with excess of orthophosphate is described. The principle of this method lies in the formation of phosphomolybdate and PPi-molybdate complexes with subsequent extraction of the phosphomolybdate complex by organic solvents and reduction of the PPi-molybdate complex by dithiothreitol and Eikonogen. The sensitivity of the method was from 5 to 120 nmol of PPi in a 2.0-ml sample.Keywords
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