Kinetics of apatite formation on a calcium-silicate cement for root-end filling during ageing in physiological-like phosphate solutions
- 27 November 2009
- journal article
- Published by Springer Science and Business Media LLC in Clinical Oral Investigations
- Vol. 14 (6), 659-668
- https://doi.org/10.1007/s00784-009-0356-3
Abstract
The bioactivity of calcium silicate mineral trioxide aggregate (MTA) cements has been attributed to their ability to produce apatite in presence of phosphate-containing fluids. This study evaluated surface morphology and chemical transformations of an experimental accelerated calcium-silicate cement as a function of soaking time in different phosphate-containing solutions. Cement discs were immersed in Dulbecco’s phosphate-buffered saline (DPBS) or Hank’s balanced salt solution (HBSS) for different times (1–180 days) and analysed by scanning electron microscopy connected with an energy dispersive X-ray analysis (SEM-EDX) and micro-Raman spectroscopy. SEM-EDX revealed Ca and P peaks after 14 days in DPBS. A thin Ca- and P-rich crystalline coating layer was detected after 60 days. A thicker multilayered coating was observed after 180 days. Micro-Raman disclosed the 965-cm−1 phosphate band at 7 days only on samples stored in DPBS and later the 590- and 435-cm−1 phosphate bands. After 60–180 days, a layer ∼200–900 μm thick formed displaying the bands of carbonated apatite (at 1,077, 965, 590, 435 cm-1) and calcite (at 1,088, 713, 280 cm−1). On HBSS-soaked, only calcite bands were observed until 90 days, and just after 180 days, a thin apatite–calcite layer appeared. Micro-Raman and SEM-EDX demonstrated the mineralization induction capacity of calcium-silicate cements (MTAs and Portland cements) with the formation of apatite after 7 days in DPBS. Longer time is necessary to observe bioactivity when cements are immersed in HBSS.Keywords
This publication has 49 references indexed in Scilit:
- Histologic assessment of Mineral Trioxide Aggregate as a root-end filling in monkeysInternational Endodontic Journal, 2009
- Histological, ultrastructural and quantitative investigations on the response of healthy human pulps to experimental capping with Mineral Trioxide Aggregate: a randomized controlled trial¶International Endodontic Journal, 2009
- Calcium silicate coating derived from Portland cement as treatment for hypersensitive dentineJournal of Dentistry, 2008
- Aspects of the in vitro bioactivity of hydraulic calcium (alumino)silicate cementJournal of Biomedical Materials Research Part A, 2008
- Innovative silicate‐based cements for endodontics: A study of osteoblast‐like cell responseJournal of Biomedical Materials Research Part A, 2008
- Guided tissue remineralisation of partially demineralised human dentineBiomaterials, 2007
- How useful is SBF in predicting in vivo bone bioactivity?Biomaterials, 2006
- Hydration of tricalcium aluminate (C3A) in the presence and absence of gypsum—studied by Raman spectroscopy and X-ray diffractionJournal of Materials Chemistry, 2006
- Micro‐Raman spectroscopy in white portland cement hydration: long‐term study at room temperatureJournal of Raman Spectroscopy, 2005
- The bonding behavior of calcite to boneJournal of Biomedical Materials Research, 1991