Short‐term antibacterial activity of root canal sealers towards Enterococcus faecalis
- 7 June 2005
- journal article
- Published by Wiley in International Endodontic Journal
- Vol. 38 (7), 483-488
- https://doi.org/10.1111/j.1365-2591.2005.00981.x
Abstract
Aim To investigate the antimicrobial activity of root canal sealers on Enterococcus faecalis, either allowing or avoiding direct contact between sealers and bacteria. Methodology Filter paper discs were immersed in standardized E. faecalis suspensions and exposed to freshly mixed sealers (MCS, AH Plus, Grossman's sealer, Sealapex, Apexit) in teflon wells for 30 min, with or without a filter membrane placed between filter paper discs and sealers (membrane-restricted contact test and direct contact test, respectively). After exposure, the filter paper discs were transferred to vials containing phosphate-buffered saline (PBS) and glass beads, and vigorously vortexed. PBS with resuspended bacterial cells was serially diluted and 25 μL droplets were seeded on TSA plates. The plates were incubated in air at 37 °C for 24 h and colony-forming units were counted. Using α = 0.05 as level for statistical significance, the data obtained were analysed using Student's t-test. Results In the direct contact test, MCS and AH Plus killed the bacteria to a level below the detection limit. They were followed in decreasing order of efficacy by Grossman's sealer, Sealapex and Apexit. In the membrane-restricted contact test, the sealers ranked: MCS, AH Plus, Grossman's sealer, Apexit and Sealapex, in descending order of antibacterial potency. MCS, AH Plus and Grossman's sealer significantly reduced the number of viable bacteria in both tests. Sealapex and Apexit were not statistically different from control. Conclusions MCS, AH Plus and Grossman's sealer were effective in reducing the number of cultivable cells of E. faecalis. Calcium hydroxide-based sealers, Sealapex and Apexit were ineffective in this short-term experiment.Keywords
This publication has 34 references indexed in Scilit:
- Influence of smear layer on depth of penetration of three endodontic sealers: an SEM studyDental Traumatology, 2007
- Survival ofEnterococcus faecalisin infected dentinal tubules after root canal filling with different root canal sealersin vitroInternational Endodontic Journal, 2004
- Inactivation of root canal medicaments by dentine, hydroxylapatite and bovine serum albuminInternational Endodontic Journal, 2001
- Antibacterial activity of calcium hydroxide‐containing endodontic sealers onEnterococcus faecalis in vitroInternational Endodontic Journal, 1997
- Biological aspects of root canal filling materials - histocompatibility, cytotoxicity, and mutagenicityClinical Oral Investigations, 1997
- The antimicrobial activity of endodontic sealers to anaerobic bacteriaInternational Endodontic Journal, 1996
- Effects of extensive apical reaming and calcium hydroxide dressing on bacterial infection during treatment of apical periodontitis: a pilot studyInternational Endodontic Journal, 1991
- Predominant obligate anaerobes invading the deep layers of root canal dentineInternational Endodontic Journal, 1990
- In vitro Infection and of Dentinal TubulesJournal of Dental Research, 1987
- The antibacterial effect of camphorated paramonochlorophenol, camphorated phenol and calcium hydroxide in the treatment of infected root canalsDental Traumatology, 1985