WRKY transcription factors WRKY12 and WRKY13 interact with SPL10 to modulate age-mediated flowering
- 31 October 2020
- journal article
- research article
- Published by Wiley in Journal of Integrative Plant Biology
- Vol. 62 (11), 1659-1673
- https://doi.org/10.1111/jipb.12946
Abstract
WRKY12 and WRKY13 are two WRKY transcription factors that play important roles in the control of flowering time under short-day (SD) conditions. The temporally regulated expression ofWRKY12andWRKY13indicates that they may be involved in the age-mediated flowering pathway. However, their roles in this pathway are poorly understood. Here, we show that the transcription ofWRKY12andWRKY13is directly regulated by SQUAMOSA PROMOTER BINDING-LIKE 10 (SPL10), a transcription factor downstream of the age pathway. Binding and activation analyses revealed that SPL10 functions as a positive regulator ofWRKY12and a negative regulator ofWRKY13. Further mechanistic investigation revealed that WRKY12 and WRKY13 physically interact with SPL10 and that both of them bind to the promoter ofmiR172b. Thus, the WRKY12-SPL10 and WRKY13-SPL10 interactions facilitate and inhibit SPL10 transcriptional function, respectively, to regulatemiR172bexpression. Together, our results show that WRKY12 and WRKY13 participate in the control of age-mediated flowering under SD conditions though physically interacting with SPLs and co-regulating the target genemiR172b.Keywords
Funding Information
- National Natural Science Foundation of China (31801023, 31671274)
- Youth Innovation Promotion Association of the Chinese Academy of Sciences
This publication has 47 references indexed in Scilit:
- Arabidopsis VQ10 interacts with WRKY8 to modulate basal defense against Botrytis cinereaJournal of Integrative Plant Biology, 2018
- Arabidopsis WRKY45 Interacts with the DELLA Protein RGL1 to Positively Regulate Age-Triggered Leaf SenescenceMolecular Plant, 2017
- WRKY8 transcription factor functions in the TMV-cg defense response by mediating both abscisic acid and ethylene signaling in ArabidopsisProceedings of the National Academy of Sciences of the United States of America, 2013
- SnapShot: Control of Flowering in ArabidopsisCell, 2010
- Seasonal and developmental timing of floweringThe Plant Journal, 2010
- Integrating hormones into the floral‐transition pathway of Arabidopsis thalianaPlant, Cell & Environment, 2009
- MicroRNAs: Target Recognition and Regulatory FunctionsCell, 2009
- Evolution of plant microRNAs and their targetsTrends in Plant Science, 2008
- The WRKY superfamily of plant transcription factorsTrends in Plant Science, 2000
- Functional analysis of the Arabidopsis thaliana SBP‐box gene SPL3: a novel gene involved in the floral transitionThe Plant Journal, 1997