Characterization of the elemental composition of newborn blood spots using sector-field inductively coupled plasma-mass spectrometry
Open Access
- 30 June 2010
- journal article
- research article
- Published by Springer Science and Business Media LLC in Journal of Exposure Science & Environmental Epidemiology
- Vol. 21 (4), 355-364
- https://doi.org/10.1038/jes.2010.19
Abstract
We developed extraction and analysis protocols for element detection in neonatal blood spots (NBSs) using sector-field inductively coupled plasma-mass spectrometry (SF-ICP-MS). A 5% (v/v) nitric acid element extraction protocol was optimized and used to simultaneously measure 28 elements in NBS card filter paper and 150 NBSs. NBS element concentrations were corrected for filter paper background contributions estimated from measurements in samples obtained from either unspotted or spotted NBS cards. A lower 95% uncertainty limit (UL) that accounted for ICP-MS method, filter paper element concentration, and element recovery uncertainties was calculated by standard methods for each individual's NBS element concentration. Filter paper median element levels were highly variable within and between lots for most elements. After accounting for measurement uncertainties, 11 elements (Ca, Cs, Cu, Fe, K, Mg, Na, P, Rb, S, and Zn) had lower 95% ULs>0 ng/spot with estimated concentrations ranging from 0.05 to >50,000 ng/spot in ≥50% of NBS samples in both correction methods. In a NBS sample minority, Li, Cd, Cs, Cr, Ni, Mo, and Pb had estimated concentrations ≥20-fold higher than the respective median level. Taking measurement uncertainties into account, this assay could be used for semiquantitative newborn blood element measurement and for the detection of individuals exposed to supraphysiologic levels of some trace elements. Adequate control of filter paper element contributions remains the primary obstacle to fully quantitative element measurement in newborn blood using NBSs.Keywords
This publication has 38 references indexed in Scilit:
- Dried blood spot sampling in combination with LC‐MS/MS for quantitative analysis of small moleculesBiomedical Chromatography, 2009
- Urinary Lead Exposure and Breast Cancer Risk in a Population-Based Case-Control StudyCancer Epidemiology, Biomarkers & Prevention, 2008
- Pilot study for utilization of dried blood spots for screening of lead, mercury and cadmium in newbornsJournal of Exposure Science & Environmental Epidemiology, 2008
- Meeting Report: The Use of Newborn Blood Spots in Environmental Research: Opportunities and ChallengesEnvironmental Health Perspectives, 2007
- Predictors of urinary cadmium levels in adult femalesScience of The Total Environment, 2007
- Evaluation of methods for trace‐element determination with emphasis on their usability in the clinical routine laboratoryScandinavian Journal of Clinical and Laboratory Investigation, 2007
- Biomonitoring of 37 trace elements in blood samples from inhabitants of northern Germany by ICP–MSJournal of Trace Elements in Medicine and Biology, 2006
- Quantification of trace elements by sector field inductively coupled plasma mass spectrometry in urine, serum, blood and cerebrospinal fluid of patients with Parkinson's diseaseSpectrochimica Acta Part B: Atomic Spectroscopy, 2004
- Low-Level Lead Exposure and Children′s IQ: A Metaanalysis and Search for a ThresholdEnvironmental Research, 1994
- The paper punched disc technique for lead in blood samples with abnormal haemoglobin values.Occupational and Environmental Medicine, 1978