Incorporation of branched-chain fatty acid into cellular lipids and caspase-independent apoptosis in human breast cancer cell line, SKBR-3
Open Access
- 23 November 2005
- journal article
- Published by Springer Science and Business Media LLC in Lipids in Health and Disease
- Vol. 4 (1), 29
- https://doi.org/10.1186/1476-511x-4-29
Abstract
Background 13-Methyltetradecanoic acid (13-MTD), an iso-C15 branched- chain saturated fatty acid, has been shown to induce apoptotic cell death of numerous human cancer cells. However, the mechanism for the induction of apoptosis has not been fully understood. This study described the incorporation of 13-MTD into cellular lipid of SKBR-3 breast cancer cells and apoptosis related event to gain more insight into the mechanism action of this fatty acid. Results Treatment of SKBR-3 cells with 13-MTD lowered the cell viability and induced apoptosis. Proportion of 13-MTD in the glycerolipids increased to saturation level within 6 hours. Triacylglycerol contained 13-MTD in higher concentration than phospholipid with positional preference to sn-2. 13-MTD caused no changes in the caspase activity and its gene expression. Furthermore, addition of caspase-inhibitor to culture medium did not prevent the cells from the cytotoxicity of 13-MTD. No-increase in the cellular calcium level was also noted with 13-MTD treatment. However, 13-MTD disrupted the mitochondrial integrity in 4 hours, and increased the nuclear translocation of apoptosis inducing factor. Conclusion These results showed that 13-MTD disrupted the mitochondrial integrity, and induced apoptosis via caspase-independent death pathway.Keywords
This publication has 48 references indexed in Scilit:
- Conjugated linoleic acid induces apoptosis in MDA-MB-231 breast cancer cells through ERK/MAPK signalling and mitochondrial pathwayCancer Letters, 2006
- Apoptosis-inducing factor determines the chemoresistance of non-small-cell lung carcinomasOncogene, 2004
- Mitochondrial release of apoptosis-inducing factor occurs downstream of cytochrome c release in response to several proapoptotic stimuliThe Journal of cell biology, 2002
- Identification of Omi/HtrA2 as a Mitochondrial Apoptotic Serine Protease That Disrupts Inhibitor of Apoptosis Protein-Caspase InteractionOnline Journal of Public Health Informatics, 2002
- Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT MethodMethods, 2001
- Apoptosis and karyogamy in syncytia induced by the HIV-1-envelope glycoprotein complexCell Death & Differentiation, 2000
- Caspase-Dependent and -Independent Events in Apoptosis Induced by Hydrogen PeroxideExperimental Cell Research, 2000
- Further Evidence for Both Functional and Structural Microcompartmentation within the Membranes of Two Associated Organelles, Mitochondrion and Endoplasmic ReticulumBiochemical and Biophysical Research Communications, 1993
- Prognostic significance of tumor phosphatidylcholine stearic acid level in breast carcinomaBreast Cancer Research and Treatment, 1991
- Further characterization of mitochondrial contact sites: Effect of short-chain alcohols on membrane fluidity and activityBiochemical and Biophysical Research Communications, 1990