Recent Emergence of Insensitive Acetylcholinesterase in Chinese Populations of the Mosquito Culex pipiens (Diptera: Culicidae)
- 1 September 2006
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Medical Entomology
- Vol. 43 (5), 878-883
- https://doi.org/10.1603/0022-2585(2006)43[878:reoiai]2.0.co;2
Abstract
Organophosphate/carbamate target resistance has emerged in Culex pipiens L. (Diptera: Culicidae), the vector of Wuchereria bancrofti and West Nile virus (family Flaviviridae, genus Flavivirus) in China. The insensitive acetylcholinesterase was detected in only one of 20 samples collected on a north-to-south transect. According to previous findings, a unique mutation, G119S in the ace-1 gene, explained this high insensitivity. Phylogenetic analysis indicates that the mutation G119S recently detected in China results from an independent mutation event. The G119S mutation thus occurred at least three times independently within the Cx. pipiens complex, once in the temperate (Cx. p. pipiens) and twice in the tropical form (Cx. p. quinquefasciatus). Bioassays performed with a purified G119S strain indicated that this substitution was associated with high levels of resistance to chlorpyrifos, fenitrothion, malathion, and parathion, but low levels of resistance to dichlorvos, trichlorfon, and fenthion. Hence, it is possible that in China, dichlorvos, trichlorfon, and fenthion will still achieve effective control even in the presence of the G119S mutation.Keywords
This publication has 24 references indexed in Scilit:
- Are there non-catalytic functions of acetylcholinesterases? Lessons from mutant animal modelsBioEssays, 2005
- The molecular basis of insecticide resistance in mosquitoesInsect Biochemistry and Molecular Biology, 2004
- Insecticide resistance genes induce a mating competition cost in Culex pipiens mosquitoesGenetics Research, 2002
- Comparative linkage map development and identification of an autosomal locus for insensitive acetylcholinesterase‐mediated insecticide resistance in Culex tritaeniorhynchusInsect Molecular Biology, 2001
- Structural roles of acetylcholinesterase variants in biology and pathologyEuropean Journal of Biochemistry, 1999
- Appearance and Sweep of a Gene Duplication: Adaptive Response and Potential for New Functions in the Mosquito Culex pipiensEvolution, 1998
- A sex‐linked Ace gene, not linked to insensitive acetylcholinesterase‐mediated insecticide resistance in Culex pipiensInsect Molecular Biology, 1998
- Existence of Two Acetylcholinesterases in the Mosquito Culex pipiens (Diptera: Culicidae)Journal of Neurochemistry, 1996
- Determination ofAce.1Genotypes in Single Mosquitoes: Toward an Ecumenical Biochemical TestPesticide Biochemistry and Physiology, 1996
- Modification of acetylcholinesterase as a mechanism of resistance to insecticidesComparative Biochemistry and Physiology Part C: Pharmacology, Toxicology and Endocrinology, 1994