Polyadenylation and degradation of incomplete RNA polymerase I transcripts in mammalian cells
Open Access
- 8 January 2010
- journal article
- Published by Springer Science and Business Media LLC in EMBO Reports
- Vol. 11 (2), 106-111
- https://doi.org/10.1038/embor.2009.271
Abstract
Most transcripts in growing cells are ribosomal RNA precursors (pre‐rRNA). Here, we show that in mammals, aberrant pre‐rRNA transcripts generated by RNA polymerase I (Pol I) are polyadenylated and accumulate markedly after treatment with low concentrations of actinomycin D (ActD), which blocks the synthesis of full‐length rRNA. The poly(A) polymerase‐associated domain‐containing protein 5 is required for polyadenylation, whereas the exosome is partly responsible for the degradation of the short aberrant transcripts. Thus, polyadenylation functions in the quality control of Pol I transcription in metazoan cells. The impact of excessive aberrant RNAs on the degradation machinery is an unrecognized mechanism that might contribute to biological properties of ActD.Keywords
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