Spike mutation D614G alters SARS-CoV-2 fitness
- 26 October 2020
- journal article
- research article
- Published by Springer Science and Business Media LLC in Nature
- Vol. 592 (7852), 116-121
- https://doi.org/10.1038/s41586-020-2895-3
Abstract
A spike protein mutation D614G became dominant in SARS-CoV-2 during the COVID-19 pandemic1,2. However, the impact on viral spread and vaccine efficacy remains to be defined. Here, we engineer the D614G mutation in the USA-WA1/2020 strain and characterize its effect. D614G enhances replication on human lung epithelial cells and primary human airway tissues through an improved infectivity of virions. Hamsters infected with the G614 variant produced higher infectious titers in the nasal washes and trachea, but not lungs, confirming clinical evidence that the D614G mutation enhances viral loads in the upper respiratory tract of COVID-19 patients and may increases transmission. Sera from D614-infected hamsters exhibit modestly higher neutralization titers against G614 virus than against D614 virus, indicating that (i) the mutation may not reduce the ability of vaccines in clinical trials to protect against COVID-19 and (ii) therapeutic antibodies should be tested against the circulating G614 virus. Together with clinical findings, our work underscores the importance of this mutation in viral spread, vaccine efficacy, and antibody therapy.Keywords
This publication has 40 references indexed in Scilit:
- Prevalidation of an Acute Inhalation Toxicity Test Using the EpiAirwayIn VitroHuman Airway ModelApplied In Vitro Toxicology, 2018
- Genetic Drift during Systemic Arbovirus Infection of Mosquito Vectors Leads to Decreased Relative Fitness during Host SwitchingCell Host & Microbe, 2016
- A Comparison of Methods to Measure Fitness in Escherichia coliPLOS ONE, 2015
- Phenotypic Responses of Differentiated Asthmatic Human Airway Epithelial Cultures to RhinovirusPLOS ONE, 2015
- The New StatisticsPsychological Science, 2013
- Coronaviruses Lacking Exoribonuclease Activity Are Susceptible to Lethal Mutagenesis: Evidence for Proofreading and Potential TherapeuticsPLoS Pathogens, 2013
- Host Alternation of Chikungunya Virus Increases Fitness while Restricting Population Diversity and Adaptability to Novel Selective PressuresJournal of Virology, 2011
- Inferring relative proportions of DNA variants from sequencing electropherogramsBioinformatics, 2009
- Cross-host evolution of severe acute respiratory syndrome coronavirus in palm civet and humanProceedings of the National Academy of Sciences of the United States of America, 2005
- Clinical anatomy of the European hamster : Cricetus cricetus, LPublished by Biodiversity Heritage Library ,1978