Studies on the optimization of growth and indole alkaloid production by hairy root cultures of Catharanthus roseus
- 25 March 1991
- journal article
- Published by Wiley in Biotechnology & Bioengineering
- Vol. 37 (7), 673-680
- https://doi.org/10.1002/bit.260370709
Abstract
Catharanthus roseus hairy root cultures, genetically transformed with Agrobacterium rhizogenes, produce a wide variety of indole alkaloids. The effect of sucrose, phosphate, nitrate, and ammonia concentrations on growth and indole alkaloid production of C. roseus hairy root cultures were studied by using statistical experimental designs and linear regression analysis. Contradictory effects of these nutrients on growth and indole alkaloid production were found. The maximal growth was obtained by having 77. 8 mg NaH2PO4 · H2O/L and 1. 311 g KNO3/L in the medium, whereas the specific production of alkaloids was highest at the lowest levels of all the nutrients studied. The maximal dry weight was obtained with high values of sucrose and ammonia, but clear optimum concentrations could not be found. When having enough nutrients to support reasonable growth, it appeared difficult to affect the specific alkaloid production rates considerably. The growth (dry wt.) with the optimized nutrient concentrations in the medium was more than 50% better than in the control medium with about the same alkaloid production.Keywords
This publication has 14 references indexed in Scilit:
- Genetic transformation ofCatharanthus roseus G. Don byAgrobacterium rhizogenesPlant Cell Reports, 1989
- Thiarubrine Accumulation in Hairy Root Cultures of Chaenactis douglasiiJournal of Plant Physiology, 1988
- In situ adsorption for enhanced alkaloid production byCatharanthus roseusBiotechnology Letters, 1988
- Determination of Catharanthus alkaloids by reversed-phase high-performance liquid chromatographyJournal of Chromatography A, 1987
- Scopolamine Production by Root Cultures of Duboisia myoporoidesJournal of Plant Physiology, 1987
- Production of Hyoscyamine by ‘Hairy Root’ Cultures ofDatura stramoniumPlanta Medica, 1987
- Production of tropane alkaloids by hairy root cultures of Scopolia japonica.Agricultural and Biological Chemistry, 1986
- Metabolic Relationships of Putrescine, GABA and Alkaloids in Cell and Root Cultures of SolanaceaeProceedings in Life Sciences, 1985
- Nutrient requirements of suspension cultures of soybean root cellsExperimental Cell Research, 1968
- A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue CulturesPhysiologia Plantarum, 1962