Comparison of the protective effects of steamed and cooked broccolis on ischaemia–reperfusion-induced cardiac injury
Open Access
- 27 October 2009
- journal article
- research article
- Published by Cambridge University Press (CUP) in British Journal of Nutrition
- Vol. 103 (6), 815-823
- https://doi.org/10.1017/s0007114509992492
Abstract
Recently, broccoli, a vegetable of the Brassica family, has been found to protect the myocardium from ischaemic reperfusion injury through the redox signalling of sulphoraphane, which is being formed from glucosinolate present in this vegetable. Since cooked broccoli loses most of its glucosinolate, we assumed that fresh broccoli could be a superior cardioprotective agent compared to cooked broccoli. To test this, two groups of rats were fed with fresh (steamed) broccoli or cooked broccoli for 30 d, while a third group was given vehicle only for the same period of time. After 30 d, all the rats were sacrificed, and the isolated working hearts were subjected to 30 min ischaemia followed by 2 h of reperfusion. Both cooked and steamed broccolis displayed significantly improved post-ischaemic ventricular function and reduced myocardial infarction and cardiomyocyte apoptosis compared to control, but steamed broccoli showed superior cardioprotective abilities compared with the cooked broccoli. Corroborating with these results, both cooked and steamed broccolis demonstrated significantly enhanced induction of the survival signalling proteins including Bcl2, Akt, extracellular signal-regulated kinase 1/2, haemoxygenase-1, NFE2 related factor 2, superoxide dismutase (SOD1) and SOD2 and down-regulation of the proteins (e.g. Bax, c-Jun N-terminal kinase, p38 mitogen-activated protein kinase) of the death signalling pathway, steamed broccoli displaying superior results over its cooked counterpart. The expressions of proteins of the thioredoxin (Trx) superfamily including Trx1 and its precursor sulphoraphane, Trx2 and Trx reductase, were enhanced only in the steamed broccoli group. The results of the present study documented superior cardioprotective properties of the steamed broccoli over cooked broccoli because of the ability of fresh broccoli to perform redox signalling of Trx.Keywords
This publication has 39 references indexed in Scilit:
- Sulforaphane but not ascorbigen, indole‐3‐carbinole and ascorbic acid activates the transcription factor Nrf2 and induces phase‐2 and antioxidant enzymes in human keratinocytes in cultureExperimental Dermatology, 2010
- Activation of NF-E2–Related Factor-2 Reverses Biochemical Dysfunction of Endothelial Cells Induced by Hyperglycemia Linked to Vascular DiseaseDiabetes, 2008
- Cardioprotection with α-tocopheryl phosphate: Amelioration of myocardial ischemia reperfusion injury is linked with its ability to generate a survival signal through Akt activationBiochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, 2008
- Broccoli (Brassica oleraceavar. botrytis L.) improves the survival and up‐regulates endogenous antioxidant enzymes inDrosophila melanogasterchallenged with reactive oxygen speciesJournal of the Science of Food and Agriculture, 2007
- Glucoraphanin, the bioprecursor of the widely extolled chemopreventive agent sulforaphane found in broccoli, induces Phase-I xenobiotic metabolizing enzymes and increases free radical generation in rat liverMutation Research, 2006
- Correlation Analyses of Phytochemical Composition, Chemical, and Cellular Measures of Antioxidant Activity of Broccoli (Brassica oleraceaL. Var. italica)Journal of Agricultural and Food Chemistry, 2005
- Textural change and antioxidant properties of broccoli under different cooking treatmentsFood Chemistry, 2005
- Traditional phytotherapy in Central Italy (Marche, Abruzzo, and Latium)Fitoterapia, 2004
- Variation in content of bioactive components in broccoliJournal of Food Composition and Analysis, 2003
- Reduction of Cancer Risk by Consumption of Selenium-Enriched Plants: Enrichment of Broccoli with Selenium Increases the Anticarcinogenic Properties of BroccoliJournal of Medicinal Food, 2003