In situ visualization and dynamics of newly synthesized proteins in rat hippocampal neurons

Abstract

Dieterich et al. describe a methodology to label all newly synthesized neuronal proteins in situ. This method, which they name FUNCAT, relies on the inclusion of noncanonical amino acids and selective fluorescent labeling via click chemistry. The authors show that this system is amenable to dual pulse-chase experiments and dynamic tracking of newly synthesized proteins. Protein translation has been implicated in different forms of synaptic plasticity, but direct in situ visualization of new proteins is limited to one or two proteins at a time. Here we describe a metabolic labeling approach based on incorporation of noncanonical amino acids into proteins followed by chemoselective fluorescence tagging by means of 'click chemistry'. After a brief incubation with azidohomoalanine or homopropargylglycine, a robust fluorescent signal was detected in somata and dendrites. Pulse-chase application of azidohomoalanine and homopropargylglycine allowed visualization of proteins synthesized in two sequential time periods. This technique can be used to detect changes in protein synthesis and to evaluate the fate of proteins synthesized in different cellular compartments. Moreover, using strain-promoted cycloaddition, we explored the dynamics of newly synthesized membrane proteins using single-particle tracking and quantum dots. The newly synthesized proteins showed a broad range of diffusive behaviors, as would be expected for a pool of labeled proteins that is heterogeneous.