A comparative study of the quantitative accuracy of three‐dimensional reconstructions of spinal cord from serial histological sections
- 12 May 2003
- journal article
- research article
- Published by Wiley in Journal of Microscopy
- Vol. 210 (2), 138-148
- https://doi.org/10.1046/j.1365-2818.2003.01130.x
Abstract
We evaluated the accuracy of estimating the volume of biological soft tissues from their three‐dimensional (3D) computer wireframe models, reconstructed from histological data sets obtained from guinea‐pig spinal cords. We compared quantification from two methods of three‐dimensional surface reconstruction to standard quantitative techniques, Cavalieri method employing planimetry and point counting and Geometric Best‐Fitting. This involved measuring a group of spinal cord segments and test objects to evaluate the accuracy of our novel quantification approaches. Once a quantitative methodology was standardized there was no statistical difference in volume measurement of spinal segments between quantification methods. We found that our 3D surface reconstructions’ ability to model precisely actual soft tissues provided an accurate volume quantification of complex anatomical structures as standard approaches of Cavalieri estimation and Geometric Best‐Fitting. Additionally, 3D reconstruction quantitatively interrogates and three‐dimensionally images spinal cord segments and obscured internal pathological features with approximately the same effort required for standard quantification alone.Keywords
This publication has 22 references indexed in Scilit:
- New approximations for the variance in Cavalieri samplingJournal of Microscopy, 2000
- Tetrahedral meshes from planar cross-sectionsComputer Methods in Applied Mechanics and Engineering, 1999
- Precision of systematic sampling and transitive methodsJournal of Statistical Planning and Inference, 1999
- Estimation of fetal volume by magnetic resonance imaging and stereologyThe British Journal of Radiology, 1994
- Unbiased estimation of human body composition by the Cavalieri method using magnetic resonance imagingJournal of Microscopy, 1993
- Three-Dimensional Computer-Assisted Analysis of Graded Contusion Lesions in the Spinal Cord of the RatJournal of Neurotrauma, 1991
- On the precision of systematic sampling: a review of Matheron's transitive methodsJournal of Microscopy, 1989
- The accuracy of one‐dimensional systematic samplingJournal of Microscopy, 1989
- The efficiency of systematic sampling in stereology and its prediction*Journal of Microscopy, 1987
- Spinal cord contusion in the rat: Morphometric analyses of alterations in the spinal cord☆Experimental Neurology, 1985