Determination of serum tetranectin: technical and clinical evaluation of three sandwich immunoassays
- 10 August 1998
- journal article
- research article
- Published by Elsevier BV in Clinica Chimica Acta; International Journal of Clinical Chemistry
- Vol. 276 (1), 19-34
- https://doi.org/10.1016/s0009-8981(98)00092-8
Abstract
The performance of two sandwich-type immunoassays for the determination of the tumour marker tetranectin using monoclonal antibodies Hyb 130-13 and 130-14 as catching layer was compared with the performance of a polyclonal assay. Sensitivities were 0.4–0.6 μg/l, and intra- and inter-assay coefficients of variation were PPP<0.05). In sera from patients with ovarian cancer, tetranectin concentrations were lower in the polyclonal assay than in the monoclonal assays. This could, hypothetically, be explained by ligand-binding or other conformational changes in tetranectin, influencing the antigenicity of the molecule.Keywords
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