The application of on-chip optofluidic microscopy for imaging Giardia lamblia trophozoites and cysts
- 14 April 2009
- journal article
- research article
- Published by Springer Science and Business Media LLC in Biomedical Microdevices
- Vol. 11 (5), 951-958
- https://doi.org/10.1007/s10544-009-9312-x
Abstract
The optofluidic microscope (OFM) is a lensless, low-cost and highly compact on-chip device that can enable high-resolution microscopy imaging. The OFM performs imaging by flowing/scanning the target objects across a slanted hole array; by measuring the time-varying light transmission changes through the holes, we can then render images of the target objects at a resolution that is comparable to the holes’ size. This paper reports the adaptation of the OFM for imaging Giardia lamblia trophozoites and cysts, a disease-causing parasite species that is commonly found in poor-quality water sources. We also describe our study of the impact of pressure-based flow and DC electrokinetic-based flow in controlling the flow motion of Giardia cysts—rotation-free translation of the parasite is important for good OFM image acquisition. Finally, we report the successful microscopy imaging of both Giardia trophozoites and cysts with an OFM that has a focal plane resolution of 0.8 microns.Keywords
This publication has 18 references indexed in Scilit:
- Lensfree holographic imaging for on-chip cytometry and diagnosticsLab on a Chip, 2008
- Lensless high-resolution on-chip optofluidic microscopes for Caenorhabditis elegans and cell imagingProceedings of the National Academy of Sciences of the United States of America, 2008
- Enhanced microfiltration devices configured with hydrodynamic trapping and a rain drop bypass filtering architecture for microbial cells detectionLab on a Chip, 2008
- Ultra wide-field lens-free monitoring of cells on-chipLab on a Chip, 2007
- High-throughput microscopy must re-invent the microscope rather than speed up its functionsBritish Journal of Pharmacology, 2007
- Lab-on-a-chip devices for microbial monitoring and detection in waterWater Supply, 2007
- Microbial detection in microfluidic devices through dual staining of quantum dots-labeled immunoassay and RNA hybridizationAnalytica Chimica Acta, 2006
- Focused electrophoretic motion and selected electrokinetic dispensing of particles and cells in cross-microchannelsElectrophoresis, 2005
- Filter-based microfluidic device as a platform for immunofluorescent assay of microbial cellsLab on a Chip, 2004
- A passive pumping method for microfluidic devicesLab on a Chip, 2002