Characterization of Flavins in Roots of Fe-Deficient Strategy I Plants, with a Focus on Medicago truncatula
Open Access
- 28 October 2011
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant and Cell Physiology
- Vol. 52 (12), 2173-2189
- https://doi.org/10.1093/pcp/pcr149
Abstract
The root accumulation and excretion of riboflavin (Rbfl) and Rbfl derivatives have been studied in the model legume species Medicago truncatula, grown in hydroponics in two different Fe deficiency conditions, with and without CaCO3. Using high resolution mass spectrometry techniques coupled to liquid chromatography, three different flavin derivatives not previously reported in plants, putatively identified as 7-hydroxy-Rbfl, 7α-hydroxy-Rbfl and 7-carboxy-Rbfl, were found along with Rbfl in Fe-deficient M. truncatula roots. In the presence of CaCO3 most of the flavins were accumulated in the roots, whereas in the absence of CaCO3 there was partial export to the nutrient solution. The major flavins in roots and nutrient solution were Rbfl and 7-hydroxy-Rbfl, respectively. Flavins were located in the root cortex and epidermal cells, preferentially in a root region near the apex that also exhibited increased ferric chelate reductase (FCR) activity. Six out of 15 different species of horticultural interest showed root increases in both Rbfl (four of them also having Rbfl derivatives) and FCR. No significant correlation was found between Rbfl and either phosphoenolpyruvate carboxylase or FCR activities, whereas the latter two showed a good correlation between them. The possible roles of Rbfl and Rbfl derivatives in roots and nutrient solutions are discussed. Medicago truncatula is proposed as a model system for flavin studies.Keywords
This publication has 42 references indexed in Scilit:
- Towards a knowledge-based correction of iron chlorosisPlant Physiology and Biochemistry, 2011
- Medicago truncatula proteomicsJournal of Proteomics, 2010
- Release of plant-borne flavonoids into the rhizosphere and their role in plant nutritionPlant and Soil, 2010
- Time course induction of several key enzymes in Medicago truncatula roots in response to Fe deficiencyPlant Physiology and Biochemistry, 2009
- A gene expression atlas of the model legume Medicago truncatulaThe Plant Journal, 2008
- Organic acids and Fe deficiency: a reviewPlant and Soil, 2002
- Time-Dependent Density Functional Response Theory for MoleculesRecent Advances in Computational Chemistry, 1995
- Density-functional thermochemistry. III. The role of exact exchangeThe Journal of Chemical Physics, 1993
- IRON AND PLANT PIGMENTSPublished by Elsevier BV ,1993
- Quantitative studies of the avidity of naturally occurring substances for trace metals. 3. Pteridines, riboflavin and purinesBiochemical Journal, 1953