Comparison of Different Methods for Detecting Carbohydrate‐Deficient Transferrin
- 1 October 1994
- journal article
- research article
- Published by Wiley in Alcohol: Clinical and Experimental Research
- Vol. 18 (5), 1150-1155
- https://doi.org/10.1111/j.1530-0277.1994.tb00096.x
Abstract
Different methods for detecting carbohydrate-deficient transferrin (CDT) were compared. In addition, their efficiency for detecting alcohol abuse among men not having clinical evidence of liver disease was studied in controls (n = 26), weekend (n = 16) and daily (n = 12) heavy drinkers, and alcoholics (n = 28). Comparisons were made between anion-exchange separation of iron-saturated transferrin (Tf) by microcolumns (CDTect) and by the Fast Protein Liquid Chromatography (FPLC% and FPLC-MG), followed by double-antibody radioimmunoassay of collected fractions. Tf fractions with pl > or = 5.7 were also measured by two different isoelectric focusing (IEF) methods, followed by immunofixation (SA-IEF-CDT and IEF-CDT-TOT), the latter method being used also for detection of asialotransferrin (IEF-CDT-AS). The cut-off was 20 units/liter for CDTect, 4.4% of total Tf for SA-IEF-CDT, and the mean +2 sd of the control group for FPLC-MG (as mg/liter of Tf), FPLC-%, IEF-CDT-TOT, and IEF-CDT-AS (all as percentage of Tf). The overall accuracies (combining sensitivity and specificity) for detecting heavy drinkers of CDTect, FPLO (mg/liter), FPLC (%), SA-IEF-CDT, IEF-CDT-TOT, and IEF-CDT-AS were 63%, 59%, 61%, 74%, 57%, and 63%, respectively; for detecting alcoholics, 87%, 83%, 81%, 89%, 37%, and 76%, respectively. In conclusion, the methods were in rather good agreement with each other. Diagnostic characteristics among heavy drinkers and correlations between methods differed slightly, probably depending on the ability of different methods to separate and detect asialo-, monosialo-, and disialotransferrin.(ABSTRACT TRUNCATED AT 250 WORDS)Keywords
This publication has 22 references indexed in Scilit:
- CDT by Anion-Exchange Chromatography Followed by RIA as a Marker of Heavy Drinking Among MenAlcohol: Clinical and Experimental Research, 1993
- The analysis of human serum transferrins with the PhastSystem: Quantitation of microheterogeneityElectrophoresis, 1992
- Carbohydrate‐Deficient Transferrin (CDT) in Serum as a Possible Indicator of Heavy Drinking in Young University StudentsAlcohol: Clinical and Experimental Research, 1992
- The efficiency of a questionnaire in detecting heavy drinkersBritish Journal of Addiction, 1990
- A comparison of serum carbohydrate-deficient transferrin with other biological markers of excessive drinkingScandinavian Journal of Clinical and Laboratory Investigation, 1988
- DESIALYLATED TRANSFERRIN AS A SEROLOGICAL MARKER OF CHRONIC EXCESSIVE ALCOHOL INGESTIONThe Lancet, 1987
- Micro Anion Exchange Chromatography of Carbohydrate‐Deficient Transferrin in Serum in Relation to Alcohol Consumption (Swedish Patent8400587–5)Alcohol: Clinical and Experimental Research, 1986
- Carbohydrate Composition of Serum Transferrin in Alcoholic PatientsAlcohol: Clinical and Experimental Research, 1986
- Quantitative estimation of abnormal microheterogeneity of serum transferrin in alcoholicsPharmacology Biochemistry and Behavior, 1980
- Introduction to clinical decision makingSeminars in Nuclear Medicine, 1978