Structure/function of human type 1 3β-hydroxysteroid dehydrogenase: An intrasubunit disulfide bond in the Rossmann-fold domain and a Cys residue in the active site are critical for substrate and coenzyme utilization
- 31 October 2007
- journal article
- Published by Elsevier BV in The Journal of Steroid Biochemistry and Molecular Biology
- Vol. 107 (1-2), 80-87
- https://doi.org/10.1016/j.jsbmb.2007.02.003
Abstract
No abstract availableKeywords
This publication has 22 references indexed in Scilit:
- Rational proteomics V: Structure-based mutagenesis has revealed key residues responsible for substrate recognition and catalysis by the dehydrogenase and isomerase activities in human 3β-hydroxysteroid dehydrogenase/isomerase type 1The Journal of Steroid Biochemistry and Molecular Biology, 2006
- Structure/Function Relationships Responsible for Coenzyme Specificity and the Isomerase Activity of Human Type 1 3β-Hydroxysteroid Dehydrogenase/IsomeraseJournal of Biological Chemistry, 2003
- Site-directed mutagenesis identifies amino acid residues associated with the dehydrogenase and isomerase activities of human type I (placental) 3β-hydroxysteroid dehydrogenase/IsomeraseThe Journal of Steroid Biochemistry and Molecular Biology, 1998
- Crystal Structures of the Oxidized and Reduced Forms of UDP-galactose 4-Epimerase Isolated from Escherichia coli,Biochemistry, 1996
- An NADH-induced Conformational Change That Mediates the Sequential 3β-Hydroxysteroid Dehydrogenase/Isomerase Activities Is Supported by Affinity Labeling and the Time-dependent Activation of IsomerasePublished by Elsevier BV ,1995
- CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choiceNucleic Acids Research, 1994
- Human placental 3β-hydroxy-5-ene-steroid dehydrogenase and steroid 5→ 4-ene-isomerase: Purification from mitochondria and kinetic profiles, biophysical characterization of the purified mitochondrial and microsomal enzymesJournal of Steroid Biochemistry, 1989
- Human placental 3β-hydroxy-5-ene-steroid dehydrogenase and steroid 5 → 4-ene-isomerase: Purification from microsomes, substrate kinetics, and inhibition by product steroidsJournal of Steroid Biochemistry, 1988
- A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye BindingAnalytical Biochemistry, 1976
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976