Regulation of Caspase-3 and Bcl-2 Expression in Dalton's Lymphoma Ascites Cells by Abrin
Open Access
- 1 January 2009
- journal article
- website
- Published by Hindawi Limited in Evidence-Based Complementary and Alternative Medicine
- Vol. 6 (2), 233-238
- https://doi.org/10.1093/ecam/nem099
Abstract
The role of abrin, a toxic lectin isolated from seeds ofAbrus precatoriusLinn in inducing apoptosis in murine Dalton's Lymphoma Ascites (DLA) cells was evaluated. Abrin when incubated at the concentration of 10 ng per million DLA cells could bring about cell death as typical morphological changes with apoptosis. However, necrotic cell death dominated when a higher dose of abrin was used. DNA samples, isolated from DLA cells treated with abrin showed fragmentation. Abrin brought about induction of apoptosis by stimulating the expression of pro-apoptotic Caspase-3, at the same time blocking the expression of Bcl-2, which is an anti apoptotic gene. However, the expression of tumor suppressor gene p53 has not been observed in control and abrin-treated DLA cells. Results suggested that abrin effectively induced apoptotic changes in the tumor cells that led to cellular death.Keywords
This publication has 31 references indexed in Scilit:
- Effect of Abrin on Cell-Mediated Immune Responses in MiceImmunopharmacology and Immunotoxicology, 2006
- Plant-derived abrin-a induces apoptosis in cultured leukemic cell lines by different mechanismsToxicology and Applied Pharmacology, 2004
- Ribosome-inactivating protein and apoptosis: abrin causes cell death via mitochondrial pathway in Jurkat cellsBiochemical Journal, 2004
- Cytotoxic and anti-tumour properties of certain taxa of Umbelliferae with special reference to Centella asiatica (L.) UrbanJournal of Ethnopharmacology, 1995
- Probing the molecular program of apoptosis by cancer chemopreventive agentsJournal of Cellular Biochemistry, 1995
- Studies on the variants of the protein toxins ricin and abrinJBIC Journal of Biological Inorganic Chemistry, 1992
- Differential effects of abrin on normal and tumor cellsJournal of Cellular Physiology, 1985
- DNA and protein synthesis in normal and transformed lymphocytes exposed to abrinPharmacology Biochemistry and Behavior, 1982
- Phytohemagglutinins (Phytolectins)Annual Review of Plant Physiology, 1976
- Abrin and hurin: Two new lymphocyte mitogensCell, 1975