Sensitive in situ hybridisation technique using biotin-streptavidin-polyalkaline phosphatase complex.
Open Access
- 1 February 1987
- journal article
- research article
- Published by BMJ in Journal of Clinical Pathology
- Vol. 40 (2), 163-166
- https://doi.org/10.1136/jcp.40.2.163
Abstract
A sensitive in situ hybridisation technique, using a biotin-streptavidin-polyalkaline phosphatase complex detection system, was successfully applied to smears of fresh cultured cells, frozen sections, and formalin fixed paraffin processed tissue: the procedure was successful for DNA-DNA hybridizations using a variety of DNA probes. The detection method is rapid, reliable, and economical producing a purplish-blue precipitate at the site of hybridisation and clearly visible by low power light microscopy.Keywords
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