Effect of tetracycline residues in pig manure slurry on tetracycline-resistant bacteria and resistance gene tet(M) in soil microcosms
- 30 September 2006
- journal article
- Published by Elsevier BV in Environment International
- Vol. 32 (7), 876-882
- https://doi.org/10.1016/j.envint.2006.05.008
Abstract
Effects of tetracycline residues from pig manure slurry on the prevalence of tetracycline-resistant bacteria and the tetracycline resistance gene, tet(M), were studied in soil microcosms. Four types of soil microcosms were established for a period of 152 days, supplemented with combinations of pig manure slurry and a tetracycline-resistant Enterococcus faecalis, CG110, containing the tetracycline resistance gene tet(M) (on the conjugative transposon, Tn916). The prevalence of both tetracycline-resistant aerobic bacteria and tetracycline-resistant enterococci declined rapidly until day 45 where no significant differences in the levels of tetracycline-resistant bacteria in any of the four types of microcosms could be detected. tet(M) could be detected in microcosms supplemented with either pig manure slurry and/or E. faecalis CG110 (tet(M)) for the whole period (152 days). tet(M) could be detected longer than tetracycline-resistant enterococci could be isolated (limit of detection 100 CFU/g soil) probably due to viable but not culturable (VBNC) bacteria with tet(M), horizontal gene transfer of tet(M) to indigenous soil bacteria or presence of “free” DNA. The concentration of chlortetracycline and oxytetracycline were almost stable through out the experimental period, but the tetracycline concentrations had no effect on prevalence of tetracycline-resistant bacteria. The presented microcosm approach simulated natural farmland conditions well and supported results from previous field studies.Keywords
This publication has 15 references indexed in Scilit:
- Dissipation and effects of chlortetracycline and tylosin in two agricultural soils: A field‐scale study in southern DenmarkEnvironmental Toxicology and Chemistry, 2005
- Simultaneous extraction of tetracycline, macrolide and sulfonamide antibiotics from agricultural soils using pressurised liquid extraction, followed by solid-phase extraction and liquid chromatography–tandem mass spectrometryJournal of Chromatography A, 2004
- Development of a rapid method for direct detection of tet(M) genes in soil from Danish farmlandEnvironment International, 2004
- Occurrence, Structure, and Mobility of Tn 1546 -Like Elements in Environmental Isolates of Vancomycin-Resistant EnterococciApplied and Environmental Microbiology, 2004
- Use of oxytetracycline and tylosin in intensive calf farming: evaluation of transfer to manure and soilChemosphere, 2003
- The identification of a tetracycline resistance gene tet(M), on a Tn916-like transposon, in the Bacillus cereus groupFEMS Microbiology Letters, 2002
- Determination of Persistent Tetracycline Residues in Soil Fertilized with Liquid Manure by High-Performance Liquid Chromatography with Electrospray Ionization Tandem Mass SpectrometryAnalytical Chemistry, 2002
- Survival of faecal indicator bacteria in bovine manure incorporated into soilLetters in Applied Microbiology, 2001
- Occurrence and Diversity of Tetracycline Resistance Genes in Lagoons and Groundwater Underlying Two Swine Production FacilitiesApplied and Environmental Microbiology, 2001
- Isolation of Tn916-like conjugal elements from swine lot effluentCanadian Journal of Microbiology, 2000