Short‐term culture and γH2AX flow cytometry determine differences in individual radiosensitivity in human peripheral T lymphocytes
- 12 December 2006
- journal article
- Published by Wiley in Environmental and Molecular Mutagenesis
- Vol. 48 (1), 38-47
- https://doi.org/10.1002/em.20273
Abstract
Histone H2AX, a subfamily of histone H2A, is phosphorylated and forms proteinaceous repair foci at the sites of DNA double‐strand breaks in response to genotoxic insults, such as ionizing radiation. This process is believed to play a key role in the repair of DNA damage. In this study, we established a flow cytometry (FCM) system for measuring radiation‐induced phosphorylated histone H2AX (γH2AX) in cultured human T lymphocytes to evaluate individual radiation sensitivity in vitro. Irradiation of short‐term (∼7 days) cultured T lymphocytes exhibited significant interindividual, but not interexperimental, differences in the cellular content of γH2AX 6 hr after 4 Gy of X‐irradiation in three independent experiments using peripheral blood lymphocytes from six healthy donors. However, these differences were not as marked in uncultured lymphocytes, or lymphocytes that were cultured for a prolonged period (∼13 days). The variation of γH2AX focus formation in lymphocytes of individuals was reproducible, with differences reaching about 1.5‐fold following 7 days of culture. Therefore, the FCM‐based γH2AX measurement appeared to reflect both the temporal course and the amount of DNA damage within the irradiated lymphocytes. Further, we confirmed that the differences in residual lymphocyte subsets were not involved in individual radiosensitivity. These results suggest that the FCM‐based γH2AX assay using cultured T lymphocytes might be useful for the rapid and reliable assessment of individual radiation sensitivity involved in DNA damage repair. Environ. Mol. Mutagen., 2007.Keywords
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