Rapid deactivation of MAP kinase in PC12 cells occurs independently of induction of phosphatase MKP‐1
- 10 October 1994
- journal article
- Published by Wiley in FEBS Letters
- Vol. 353 (1), 9-12
- https://doi.org/10.1016/0014-5793(94)01000-5
Abstract
Growth factors or serum can induce transcription and translation of a dual specificity MAP (mitogen-activated protein) kinase phosphatase, MKP-1 (MAP kinase phosphatase-1). The role of induction of MKP-1 (formerly 3CH134) in the rapid phase of MAP kinase deactivation was studied in rat pheochromocytoma (PC12) cells. MAP kinase was nearly completely deactivated in PC12 cells by 10 min after stimulation with epidermal growth factor (EGF) whereas MAP kinase activity remained elevated at 30% of the maximal response after stimulation with nerve growth factor. Protocols for treating cells with actinomycin D and cycloheximide were established that eliminate detection of MKP-1 mRNA and protein in PC 12 cells. Treatment of PC12 cells with actinomycin D and cycloheximide did not affect the rapid deactivation of MAP kinase. Thus, the rapid phase of MAP kinase deactivation in PC12 cells is not dependent on the induction of the MAP kinase phosphatase MKP-1.Keywords
This publication has 18 references indexed in Scilit:
- Control of MAP kinase activation by the mitogen-induced threonine/tyrosine phosphatase PAC1Nature, 1994
- Inactivation of MAP kinasesTrends in Biochemical Sciences, 1994
- The interaction of SV40 small tumor antigen with protein phosphatase 2A stimulates the map kinase pathway and induces cell proliferationCell, 1993
- Inhibition of the EGF-Activated MAP Kinase Signaling Pathway by Adenosine 3′,5′-MonophosphateScience, 1993
- MKP-1 (3CH134), an immediate early gene product, is a dual specificity phosphatase that dephosphorylates MAP kinase in vivoCell, 1993
- Biphasic and synergistic activation of p44mapk (ERK1) by growth factors: correlation between late phase activation and mitogenicityMolecular Endocrinology, 1992
- Microtubule‐associated‐protein (MAP) kinase activated by nerve growth factor and epidermal growth factor in PC12 cellsEuropean Journal of Biochemistry, 1990
- Requirement for integration of signals from two distinct phosphorylation pathways for activation of MAP kinaseNature, 1990
- Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extractionAnalytical Biochemistry, 1987
- Expression of a set of growth-related immediate early genes in BALB/c 3T3 cells: coordinate regulation with c-fos or c-myc.Proceedings of the National Academy of Sciences of the United States of America, 1987