Toxicity of Xylocaine to rabbit corneal endothelium
- 1 October 1998
- journal article
- Published by Ovid Technologies (Wolters Kluwer Health) in Journal of Cataract & Refractive Surgery
- Vol. 24 (10), 1371-1376
- https://doi.org/10.1016/s0886-3350(98)80231-9
Abstract
Purpose: To assess the toxicity of lidocaine hydrochloride (Xylocaine®) to the corneal endothelium. Setting: Department of Ophthalmology, Hotel-Dieu Hospital, Paris, France. Methods: Rabbit corneas were excised and the endothelium was exposed to balanced salt solution (BSS®), Xylocaine 1 %, or Xylocaine 5% (5 corneas/group) for 20 minutes. The endothelium was then stained with trypan blue and alizarin red, and 5 photomicrographs were taken of each cornea at a standard magnification and analyzed with a digital imaging system (Biocom 200). Results: Xylocaine solutions produced changes in endothelial cell morphology, but there was no cell staining with trypan blue. Corneas exposed to Xylocaine 5% had more marked cell alterations. Small areas of cells were lost from all 15 corneas, mainly at the periphery, but the differences among the 3 groups of corneas were not significant. Conclusion: Exposure of rabbit corneal endothelium to Xylocaine solutions in vitro was not associated with trypan blue staining of endothelial cells.Keywords
This publication has 13 references indexed in Scilit:
- Endothelial damage caused by uncoated and fluorocarbon-coated poly(methyl methacrylate) intraocular lensesJournal of Cataract & Refractive Surgery, 1997
- A Comparison of Topical and Retrobulbar Anesthesia for Cataract SurgeryOphthalmology, 1996
- Drop, then decide approach to topical anesthesiaJournal of Cataract & Refractive Surgery, 1995
- Pinpoint anesthesia: A new approach to local ocular anesthesiaJournal of Cataract & Refractive Surgery, 1994
- A new local anesthesia technique for cataract extraction by one quadrant sub-Tenon's infiltration.British Journal of Ophthalmology, 1992
- Anterior periocular anesthesia: Five years experienceJournal of Cataract & Refractive Surgery, 1991
- Inadvertent Globe Perforation during Retrobulbar and Peribulbar AnesthesiaOphthalmology, 1991
- The Effect of Fluorouracil on the Corneal EndotheliumAmerican Journal of Ophthalmology, 1988
- Bran Stem Anesthesia after Retrobulbar BlockOphthalmology, 1987
- Administration of periocular anesthesiaJournal of Cataract & Refractive Surgery, 1986