A Polymorphism in the Hemagglutinin of the Human Isolate of a Highly Pathogenic H5N1 Influenza Virus Determines Organ Tropism in Mice
- 15 August 2010
- journal article
- research article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 84 (16), 8316-8321
- https://doi.org/10.1128/jvi.00850-10
Abstract
We characterized a human H5N1 virus isolate (KAN-1) encoding a hemagglutinin (HA) with a K-to-E substitution at amino acid position 222 that was previously described to be selected in the lung of the infected patient. In mice, the growth of the HA 222E -encoding virus was mainly confined to the lung, but reversion to 222K allowed virus to spread to the brain. The HA 222E variant showed an overall reduced binding affinity compared to that of HA 222K for synthetic Neu5Ac2-3Gal-terminated receptor analogues, except for one analogue [Neu5Acα2-3Galβ1-4(Fucα1-3)(6-HSO 3 )GlcNAcβ, Su-SLe x ]. Our results suggest that human-derived mutations in HA of H5N1 viruses can affect viral replication efficiency and organ tropism.This publication has 17 references indexed in Scilit:
- In Vitro Assessment of Attachment Pattern and Replication Efficiency of H5N1 Influenza A Viruses with Altered Receptor SpecificityJournal of Virology, 2010
- Positive selection at the receptor-binding site of haemagglutinin H5 in viral sequences derived from human tissuesJournal of General Virology, 2008
- 6-sulfo sialyl Lewis X is the common receptor determinant recognized by H5, H6, H7 and H9 influenza viruses of terrestrial poultryVirology Journal, 2008
- Receptor Specificity of Influenza Viruses and Its Alteration during Interspecies TransmissionPublished by S. Karger AG ,2008
- Evolution of the receptor binding phenotype of influenza A (H5) virusesVirology, 2006
- Productive infection in the murine central nervous system with avian influenza virus A (H5N1) after intranasal inoculationActa Neuropathologica, 2004
- A DNA transfection system for generation of influenza A virus from eight plasmidsProceedings of the National Academy of Sciences of the United States of America, 2000
- Polyacrylamide-based glycoconjugates as tools in glycobiologyGlycoconjugate Journal, 1998
- Influenza virus strains selectively recognize sialyloligosaccharides on human respiratory epithelium; the role of the host cell in selection of hemagglutinin receptor specificityVirus Research, 1993
- A solid-phase enzyme-linked assay for influenza virus receptor-binding activityJournal of Virological Methods, 1992