Effect of NRG1, GDNF, EGF and NGF in the Migration of a Schwann Cell Precursor Line
- 13 July 2010
- journal article
- research article
- Published by Springer Science and Business Media LLC in Neurochemical Research
- Vol. 35 (10), 1643-1651
- https://doi.org/10.1007/s11064-010-0225-0
Abstract
The Schwann cells are the myelinating glia of the peripheral nervous system that originated during development from the highly motile neural crest. However, we do not know what the guidance signals for the Schwann cell precursors are. Therefore, we set to test some of the known neurotrophins that are expressed early in developing embryos and have been shown to be critical for the survival and patterning of developing glia and neurons. The goal of this study was to determine more specifically if GDNF, NRG1 and NGF are chemoattractants and/or chemokinetic molecules for a Schwann cell precursor line, the Spl201. We performed live chemoattraction assays, with imaging and also presented these molecules as part of their growing substrate. Our results show for the first time that GDNF and NRG1 are potent chemoattractive and chemokinetic molecules for these cells while NGF is a chemokinetic molecule stimulating their motility.Keywords
This publication has 50 references indexed in Scilit:
- Mutant p53 Drives Invasion by Promoting Integrin RecyclingCell, 2009
- Effects of NGF, NT-3 and GDNF family members on neurite outgrowth and migration from pelvic ganglia from embryonic and newborn miceBMC Developmental Biology, 2008
- Chemotaxis in shallow gradients is mediated independently of PtdIns 3-kinase by biased choices between random protrusionsNature, 2007
- Glial cell line‐derived neurotrophic factor promotes olfactory ensheathing cells migrationGlia, 2006
- Invariant mantling of growth cones by Schwann cell precursors characterize growing peripheral nerve frontsGlia, 2006
- The Neural Cell Adhesion Molecule NCAM Is an Alternative Signaling Receptor for GDNF Family LigandsCell, 2003
- Cell migration and axon growth cone guidance in Caenorhabditis elegansCurrent Opinion in Genetics & Development, 1999
- Schwann Cells Express NDF and SMDF/n-ARIA mRNAs, Secrete Neuregulin, and Show Constitutive Activation of erbB3 Receptors: Evidence for a Neuregulin Autocrine LoopExperimental Neurology, 1997
- P0 is an early marker of the schwann cell lineage in chickensNeuron, 1991
- The nerve growth factor familyProgress in Growth Factor Research, 1990