Relation of human sperm acrosin activity and fertilization in vitro
- 24 April 2009
- journal article
- Published by Hindawi Limited in Andrologia
- Vol. 26 (1), 9-15
- https://doi.org/10.1111/j.1439-0272.1994.tb00746.x
Abstract
The aim of the study described here was to determine the possible contribution of the acrosin activity test to routine semen analysis in enhancing the precision of the prognosis of IVF success in a group of patients in which the contribution of the egg factor to infertility was ruled out (20 cases) compared to a control IVF group (39 cases). Semen analysis, acrosin activity and acrosome ultrastructure were determined for all semen samples. The group with high fertilization rates was comprised of normozoospermic patients while the group with low fertilization rates was comprised of astheno-teratozoospermic patients. The mean acrosin level of the positive IVF group was significantly higher than that of the negative group (51.7 +/- 33.2 and 28.6 +/- 13.7, respectively). Two parameters: per cent motile spermatozoa and acrosin level, were found to have a significant positive correlation with subsequent successful IVF (r = 0.36, P < 0.006; r = 0.37, P < 0.004, respectively); and agenesis of the acrosome was found to have a significant negative correlation (r = -0.33, P < 0.01). The ability of these parameters to correctly predict fertilization success was 59%, with 5% false positive, among which 15.4% was predicted solely by the acrosin level (above 54 microIU 10(6) cells-1) and 23% solely by per cent motile spermatozoa (above 50%). Abnormalities of the acrosome ultrastructure did not contribute further to the correct classification. The apparent clinical benefit of the acrosin level test is discussed.Keywords
This publication has 14 references indexed in Scilit:
- Human sperm acrosin activity with relation to semen parameters and acrosomal ultrastructureAndrologia, 2009
- The relationship between sperm ultrastructural features and fertilizing capacity in vitroFertility and Sterility, 1992
- Low acrosin activity in a subgroup of men with idiopathic infertility does not correlate with sperm density, percent motility, curvilinear velocity, or linearityFertility and Sterility, 1989
- A Simple, Clinical Assay to Evaluate The Acrosin Activity of Human SpermatozoaJournal of Andrology, 1989
- Corrective measures and pregnancy outcome in in vitro fertilization in patients with severe sperm morphology abnormalitiesFertility and Sterility, 1988
- Predictive value of abnormal sperm morphology in in vitro fertilizationFertility and Sterility, 1988
- Relationship of Human Sperm Acrosin and Proacrosin to Semen Parameters II. CorrelationsJournal of Andrology, 1987
- Sperm factors related to failure of human in-vitro fertilizationReproduction, 1986
- Relationship of Human Sperm Acrosin and Proacrosin to Semen Parameters. I. Comparisons Between Symptomatic Men of Infertile Couples and Asymptomatic Men, and Between Different Split Ejaculate FractionsJournal of Andrology, 1982
- Comparison of Several Extraction Procedures for Boar Spermatozoal Acrosin1Biology of Reproduction, 1976