BALB/c-Fcgr2b−/−Pdcd1−/− mouse expressing anti-urothelial antibody is a novel model of autoimmune cystitis
Open Access
- 19 March 2012
- journal article
- Published by Springer Science and Business Media LLC in Scientific Reports
- Vol. 2 (1), 317
- https://doi.org/10.1038/srep00317
Abstract
We report the impact of anti-urothelial autoantibody (AUAb) on urinary bladder phenotype in BALB/c mice deficient of the FcγRIIb and PD-1. AUAb was present in serum samples from approximately half of the double-knockout (DKO) mice, as detected by immunofluorescence and immunoblots for urothelial proteins including uroplakin IIIa. The AUAb-positive DKO mice showed degeneration of urothelial plaque and umbrella cells, along with infiltration of inflammatory cells in the suburothelial layer. TNFα and IL-1β were upregulated in the bladder and the urine of AUAb-positive DKO mice. Voiding behavior of mice was analyzed by the Voided Stain on Paper method. 10-week-old and older AUAb-positive DKO mice voided significantly less urine per void than did wild type (WT) mice. Furthermore, administration of the AUAb-containing serum to WT mice significantly reduced their urine volume per void. In summary, this report presents a novel comprehensive mouse model of autoimmune cystitis.This publication has 28 references indexed in Scilit:
- Autoimmunity to Uroplakin II Causes Cystitis in Mice: A Novel Model of Interstitial CystitisEuropean Urology, 2012
- RDP58 inhibits T cell-mediated bladder inflammation in an autoimmune cystitis modelJournal of Autoimmunity, 2008
- Voiding Pattern Analysis as a Surrogate for Cystometric Evaluation in Uroplakin II Knockout MiceJournal of Urology, 2008
- RANTES mediates TNF-dependent lamina propria mast cell accumulation and barrier dysfunction in neurogenic cystitisAmerican Journal of Physiology-Renal Physiology, 2007
- Sensitizing Effect of Galectin-7 in Urothelial Cancer to Cisplatin through the Accumulation of Intracellular Reactive Oxygen SpeciesCancer Research, 2007
- Hydronephrosis associated with antiurothelial and antinuclear autoantibodies in BALB/c-Fcgr2b −/− Pdcd1 −/− miceThe Journal of Experimental Medicine, 2005
- Roles of uroplakins in plaque formation, umbrella cell enlargement, and urinary tract diseasesThe Journal of cell biology, 2004
- Mast Cell Activation Triggers a Urothelial Inflammatory Response Mediated by Tumor Necrosis Factor-αJournal of Urology, 2002
- Autoimmune Dilated Cardiomyopathy in PD-1 Receptor-Deficient MiceScience, 2001
- Spontaneous Autoimmune Disease in FcγRIIB-Deficient Mice Results from Strain-Specific EpistasisImmunity, 2000