Intrinsic peroxidase-like activity of mesoporous nickel oxide for selective cysteine sensing
- 18 July 2014
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in Journal of Materials Chemistry B
- Vol. 2 (36), 6097-6105
- https://doi.org/10.1039/c4tb00968a
Abstract
Mesoporous nickel oxide nanoflowers (NiO NFs) can be easily synthesized by a two-step synthetic procedure based on modified hydrothermal (MHT) treatment of nickel acetate and ethanol amine in water followed by thermal decomposition at 350 °C for 4 h. After thermal treatment, the porosity is increased by 18% with retention of parental nickel hydroxide size. In this study, for the first time, a new catalytic application of NiO NFs has been revealed in terms of peroxidase-like activity where colorless 3,3′,5,5′ tetramethylbenzidine (TMB) is oxidized to blue color product in the presence of H2O2 at room temperature. Comparative study confirms that mesoporous NiO NFs exhibit superior catalytic activity to the parent analogues, i.e. Ni(OH)2 or bulk NiO. This intrinsic peroxidase-like activity from an easily synthesized inorganic nanomaterial provides an alternative to horseradish peroxidase (HRP) enzyme. The lower Michaelis constant (Km) value indicates that the catalyst NiO NFs bind efficiently to the test substrate, i.e. TMB. Interestingly, the NiO NFs-catalyzed TMB oxidation, i.e. blue color formation, has been found to be selectively and successively inhibited by a variable amount of cysteine among a set of 21 congeners. Thus our adopted simple, low-cost and novel colorimetric assay stands to be a highly efficient approach for selective detection of cysteine with a limit of detection (LOD) value of ∼1.1 μM using a simple UV-vis spectrophotometer. The proposed method also exhibits outstanding selectivity and accuracy for N-acetyl cysteine (an analogue of cysteine) estimation in real pharmaceutical samples.Keywords
This publication has 49 references indexed in Scilit:
- Silver Nanoparticle Decorated Reduced Graphene Oxide (rGO) Nanosheet: A Platform for SERS Based Low-Level Detection of Uranyl IonACS Applied Materials & Interfaces, 2013
- 2D ultrathin nanosheets of Co–Al layered double hydroxides prepared in l-asparagine solution: enhanced peroxidase-like activity and colorimetric detection of glucoseJournal of Materials Chemistry B, 2013
- V2O5 Nanowires with an Intrinsic Peroxidase‐Like ActivityAdvanced Functional Materials, 2010
- Ultrasensitive fluorometric determination of hydrogen peroxide and glucose by using multiferroic BiFeO3 nanoparticles as a catalystTalanta, 2010
- Prospects of Colloidal Nanocrystals for Electronic and Optoelectronic ApplicationsChemical Reviews, 2009
- Semiconductor Nanowires for Energy ConversionChemical Reviews, 2009
- Oxidase‐Like Activity of Polymer‐Coated Cerium Oxide NanoparticlesAngewandte Chemie-International Edition, 2009
- Intrinsic peroxidase-like activity of ferromagnetic nanoparticlesNature Nanotechnology, 2007
- Nanostructured materials for advanced energy conversion and storage devicesNature Materials, 2005
- Environmental Applications of Semiconductor PhotocatalysisChemical Reviews, 1995