Comparison of the Bioactive Compounds and Antioxidant Potentials of Fresh and Cooked Polish, Ukrainian, and Israeli Garlic
- 12 March 2005
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of Agricultural and Food Chemistry
- Vol. 53 (7), 2726-2732
- https://doi.org/10.1021/jf0404593
Abstract
Garlic (Allium sativum L.) is an essential part of Polish, Ukrainian, and Israeli cuisine. The aim of this investigation was to compare the changes in bioactive compounds, proteins, and antioxidant potentials in fresh Polish, Ukrainian, and Israeli garlic samples after subjection to cooking temperature. Dietary fiber and essential trace elements were comparable. The antioxidant potentials were determined by four scavenging methods using beta-carotene, 1,1-diphenyl-2-picrylhydrazyl (DPPH), nitric oxide (NO), and 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS(*)(+)) radical cation with K(2)S(2)O(8) or MnO(2) assays. Polyphenols, tocopherols, proteins, and antioxidant potentials were higher in Polish garlic, but not significantly (P > 0.05). The SDS- and native-PAGE electrophoretic patterns of all three fresh garlic samples were without significant differences. Most of the proteins were in the molecular mass range of 24-97 kDa, and the more intensive major bands were concentrated at 50 and 12 kDa. The 50 kDa protein nearly disappears and the intensity of the 12 kDa lectin bands slightly decreases during cooking. It was observed that the bioactive compounds, antioxidant potential, and proteins in garlic decrease significantly after 20 min of cooking at 100 degrees C (P < 0.05). In conclusion, (a) the bioactive compounds, electrophoretic patterns, and antioxidant potential of fresh Polish, Ukrainian, and Israeli garlic samples are comparable; (b) garlic samples subjected to 100 degrees C during 20 min preserve their bioactive compounds, antioxidant potential, and protein profile and are comparable with fresh garlic; and (c) fresh garlic should be added to dishes cooked at 100 degrees C in the last 20 min of the cooking process.Keywords
This publication has 14 references indexed in Scilit:
- Vitamin Content and Amino Acid Composition of Pickled Garlic Processed with and without FermentationJournal of Agricultural and Food Chemistry, 2004
- Nutritional composition of commercial pickled garlicZeitschrift für Lebensmittel-Untersuchung und Forschung, 2004
- Selenium and its relationship to cancer: an updateBritish Journal of Nutrition, 2004
- Identification of Six Phenylpropanoids from Garlic Skin as Major AntioxidantsJournal of Agricultural and Food Chemistry, 2003
- Antioxidant properties of aged garlic extract: an in vitro study incorporating human low density lipoproteinLife Sciences, 2003
- Comparison of antioxidant activities of onion and garlic extracts by inhibition of lipid peroxidation and radical scavenging activityFood Chemistry, 2003
- Dose-dependent induction of endogenous antioxidants in rat heart by chronic administration of garlic.Life Sciences, 2002
- Determination of ε-N-Pyrrolylnorleucine in Fresh Food ProductsJournal of Agricultural and Food Chemistry, 1999
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970