Germline Transformation of Caenorhabditis elegans by Injection
Open Access
- 15 November 2008
- book chapter
- research article
- Published by Springer Science and Business Media LLC
- Vol. 518, 123-133
- https://doi.org/10.1007/978-1-59745-202-1_10
Abstract
Microinjection is a commonly used technique for DNA transformation in Caenorhabditis elegans. It is a powerful tool that links genetic and molecular analysis to phenotypic analysis. In this chapter we shall provide an overview of microinjection for germline transformation in worms. Our discussion will emphasize C. elegans reproductive biology, applications and protocols for carrying out microinjection in order to successfully obtain transgenic worms.Keywords
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