Comparative Effect of Melatonin and Night Light Application in Ehrlich Solid Tumor Mice

Abstract

Background: In this study, it was aimed to test the anti-tumoral effects of melatonin in vivo. In the literature review, no study was found that examined the anti-tumoral effect of melatonin at night on an experimental tumor model in vivo. Therefore, in the study, the anti-tumoral effect of melatonin was tested in solid tumor tissue formed in Swiss albino male mice. Methods: Swiss albino male mice formed Swiss albino male mice in vivo and 80 Swiss albino male mice weighing 25-30 g and aged 10-12 weeks were used. The subjects were divided into 10 groups in total (n=8). These groups are divided into two main groups as light and dark. All animals except the control group were injected intramuscularly (i.m.) with EAT to create EST in the right leg scapula of the subjects. Different doses of melatonin were injected intraperitoneally (i.p.) daily to the experimental groups. EST was created by inoculating EAT 2 days after administration of melatonin. Melatonin application was made between 18:00 and 20:00 in the evening. In addition, light groups were exposed to 580 µW fluorescent light between 02:00 and 04:00 at night. Daily food and water consumption and weight gains of all animals were followed and recorded, and the application was continued for 14 days. Cardiac blood was collected from all animals with heparinized syringes on the 15th day of the experiment and tumor tissues were removed ambuloc. At the end of the experiment, all animals were sacrificed under ether anesthesia. Results: The MDA and GSH parameters in the cardiac blood taken from the subjects were examined and the diameters of the tumor tissues were measured and examined under a light microscope. Weight change in all groups was not statistically significant (p>0.05). It was found that the increases and decreases in the MDA and GSH values of the light and dark groups were not statistically significant (p>0.05). It was determined that there was no statistically significant difference between the tumor diameters of the control light groups and the tumor diameters of the other light groups (p> 0.05). While the difference between the control of dark groups and the tumor diameters of Group K2 was not statistically significant (p>0.05), the increase in tumor diameter of the subjects of Group K3 and Group K4 was found to be statistically significant (pConclusion: As a result; in this study, which investigated the effects of melatonin on experimental EST in dark and light environments, it was revealed that it did not show any positive effect on MDA and GSH levels.