Potential Use of Quantum Dots in Flow Cytometry
Open Access
- 17 December 2008
- journal article
- research article
- Published by MDPI AG in International Journal of Molecular Sciences
- Vol. 9 (12), 2622-2638
- https://doi.org/10.3390/ijms9122622
Abstract
QDs may offer significant advantages in environmental and bead-based applications where the target cells need to be discriminated above background fluorescence. We have examined the possible applications of QDs for flow cytometric measurements (FCM) by studying their excitation - emission spectra and their binding to paramagnetic beads. We labelled beads with either QDs or a commonly-used fluorochrome (FITC) and studied their fluorescence intensity by FCM. Flow cytometric comparisons indicated that the minimum fluorophore concentration required for detection of QDs above autofluorescent background was 100-fold less than for FITC.This publication has 35 references indexed in Scilit:
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