Micropore membrane inlet mass spectrometer probes suitable for measurement of tissue surface gas tensions
- 1 April 1995
- journal article
- research article
- Published by Wiley in Journal of Mass Spectrometry
- Vol. 30 (4), 563-571
- https://doi.org/10.1002/jms.1190300407
Abstract
New membrane inlet mass spectrometer (MIMS) probes are described that feature a micropore in a flat metal surface filled with a polymer membrane (Krytox high-vacuum fluorinated grease). In vitro assessment of spatial resolution, time response, stirring effect and calibration are reported. The small membrane area for this MIMS results in spatial resolution of about 30 μm, adequate for measuring gas tensions at an exposed tissue surface in experimental studies of tissue oxygenation and tissue gas exchange. These probes offer a unique combination of minimal stirring effect and rapid response speed for measurements of gas partial pressures in the per cent range in aqueous solutions. Several gases, including oxygen, carbon dioxide, helium, sulfur hexafluoride and argon, permeate the polymer membrane to give a detectable signal, and the membrane is highly impermeable to water. Membrane dimensions were calculated from time response characteristics and the measured gas sample rate for argon. The membrane area was independently confirmed by direct assessment of spatial resolution. This MIMS is highly linear, has an excellent signal-to-noise ratio and offers practical advantages over electrode approaches to the measurement of gas tensions in aqueous solution.Keywords
This publication has 38 references indexed in Scilit:
- Effects of Isoflurane, Fentanyl, or Thiopental Anesthesia on Regional Cerebral Blood Flow and Brain Surface Po2 in the Presence of a Focal Lesion in RabbitsAnesthesia & Analgesia, 1993
- Depth profiles of pH and PO2 in the isolated brain stem-spinal cord of the neonatal ratRespiration Physiology, 1993
- Direct detection and identification of volatile organic compounds dissolved in organic solvents by reversed-phase membrane introduction tandem mass spectrometryAnalytical Chemistry, 1992
- Direct insertion membrane probe for selective introduction of organic compounds into a mass spectrometerAnalytica Chimica Acta, 1990
- A new membrane inlet for on-line monitoring of dissolved, volatile organic compounds with mass spectrometryInternational Journal of Mass Spectrometry and Ion Processes, 1990
- EFFECTS OF VASOPRESSORS ON RENAL TISSUE GAS TENSIONS DURING HEMORRHAGIC SHOCK IN DOGSCritical Care Medicine, 1988
- Cerebral Oxygen Tension in Rats during Deliberate Hypotension with Sodium Nitroprusside, 2-chloroadenosine, or Deep Isoflurane AnesthesiaAnesthesiology, 1986
- Skeletal muscle oxygen pressure fields during controlled hypotension with adenosine and sodium nitroprusside A comparative study in the rabbitActa Anaesthesiologica Scandinavica, 1986
- In situ calibration of an oxygen sensor in intervertebral discsJournal of Medical Engineering & Technology, 1986