Quantitative analysis of polybrominated diphenyl ethers in adipose tissue, human serum and foodstuff samples by gas chromatography with ion trap tandem mass spectrometry and isotope dilution
- 6 December 2005
- journal article
- research article
- Published by Wiley in Rapid Communications in Mass Spectrometry
- Vol. 20 (2), 69-74
- https://doi.org/10.1002/rcm.2264
Abstract
A method based on gas chromatography (GC) separation followed by ion trap tandem mass spectrometry detection in EI mode (ITD‐MS/MS), using isotope dilution, was developed for the determination of ten native polybrominated diphenyl ethers (PBDEs) and four 13C12‐labeled congeners in biological (fat tissue and human serum) and food samples. The highest‐mass fragment ions were used as precursor ions for those congeners with molecular ions with m/z values higher than the maximum of the instrument. In these cases (hepta‐BDEs and 13C12‐hexa‐BDEs) no fragmentation was achieved under the experimental conditions employed. Repeatability (lower than 9%) and reproducibility (lower than 13%), expressed as relative standard deviation (RSD, n = 3 and 4, respectively), were satisfactory. Similarly, the coefficient of variation (n = 4) of the isotopic ratio between the two most abundant product ions was lower than 10 and 6% for native and labeled congeners, respectively. To evaluate the feasibility of the method, the optimized isotope dilution GC/ITD‐MS/MS method was used for the quantitation of selected PBDE congeners in different samples including adipose tissue, human serum and foodstuff samples, from three inter‐laboratory comparative exercises, covering a wide range of concentrations. A solid‐phase extraction procedure, previously developed for PCB determination in small‐size bird serum samples, was successfully applied to quantification of PBDEs in 1 mL samples of human serum. Copyright © 2005 John Wiley & Sons, Ltd.Keywords
This publication has 37 references indexed in Scilit:
- Quantitative Analysis of 39 Polybrominated Diphenyl Ethers by Isotope Dilution GC/Low-Resolution MSAnalytical Chemistry, 2005
- Gas chromatography/ion trap mass spectrometry applied for the determination of polybrominated diphenyl ethers in soilRapid Communications in Mass Spectrometry, 2004
- Measurement of Selected Polybrominated Diphenyl Ethers, Polybrominated and Polychlorinated Biphenyls, and Organochlorine Pesticides in Human Serum and Milk Using Comprehensive Two-Dimensional Gas Chromatography Isotope Dilution Time-of-Flight Mass SpectrometryAnalytical Chemistry, 2004
- Development of a gas chromatography/ion trap mass spectrometry based method for the quantification of polybrominated diphenyl ethers and polychlorinated biphenyls in adipose tissueRapid Communications in Mass Spectrometry, 2004
- Simultaneous determination of PCDDs, PCDFs, PCBs and PBDEs in foodTalanta, 2004
- Metabolism in the toxicokinetics and fate of brominated flame retardants—a reviewEnvironment International, 2003
- An overview of commercially used brominated flame retardants, their applications, their use patterns in different countries/regions and possible modes of releaseEnvironment International, 2003
- Determination of brominated flame retardants, with emphasis on polybrominated diphenyl ethers (PBDEs) in environmental and human samples—a reviewEnvironment International, 2003
- Application of Multidimensional (Heart-Cut) Gas Chromatography to the Analysis of Complex Mixtures of Organic Pollutants in Environmental SamplesChimia, 2003